单细胞RNA测序揭示了驴精子发生和成熟过程中的动态转录谱。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-01-01 DOI:10.1016/j.ygeno.2024.110974

Yadan Jin, Fangdi Zhang, Ruixue Ma, Jingya Xing, Min Wang, Yujiang Sun, Guoliang Zhang

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引用次数: 0

摘要

随着对驴生产需求的增加，人们越来越关注驴的繁殖。然而，我们目前对驴生殖过程中精子发生和成熟的机制的理解仍然有限。目的：本研究旨在对毛驴精子发生和成熟过程进行全面的单细胞景观分析。方法：在本研究中，我们采用单细胞RNA测序来研究驴精子发生和成熟过程中的细胞组成、基因表达模式和调控作用。结果：CDK1、CETN3和UBE2J1的表达模式可指示驴精子发生过程中特定的生殖细胞。此外，在驴附睾主细胞中特异性鉴定出DEFB121、ELSPBP1和NPC2基因。结论：我们通过单细胞RNA测序分析了驴睾丸和附睾的细胞组成和空间分布，从而获得了单细胞分辨率的综合转录图谱。

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Single-cell RNA sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation

Introduction

With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.

Objectives

This study is to provide a comprehensive single-cell landscape analysis of spermatogenesis and maturation in donkeys.

Methods

In this study, we employed single-cell RNA sequencing to investigate cell composition, gene expression patterns, and regulatory roles during spermatogenesis and maturation in donkeys.

Results

The expression patterns of CDK1, CETN3, and UBE2J1 were found to be indicative of specific germ cells during donkey spermatogenesis. Additionally, the DEFB121, ELSPBP1, and NPC2 genes were specifically identified in the principal cells of the donkey epididymis.

Conclusions

We performed single-cell RNA sequencing to analyze the cellular composition and spatial distribution of donkey testis and epididymis, thereby generating comprehensive transcriptional atlases at the single-cell resolution.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.