IF 3.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Shanyong Yi, Tingting Mai, Ying Fang, Qishuo Tian, Shuquan Zhao
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引用次数: 0

摘要

随着异丙嗪逐渐成为一种街头毒品,异丙嗪中毒事件在全球范围内时有发生。然而,长期接触异丙嗪是否会导致非酒精性脂肪肝(NAFLD)仍是一个未知数。在本研究中,连续 28 天对大鼠腹腔注射二甲苯嗪,然后采集血清和肝组织进行分析。观察发现,40 毫克/千克组大鼠体重下降,天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)水平升高。组织病理学检查显示肝脏脂肪变性、坏死和纤维化。通过 mRNA 测序,发现 40 mg/kg 组有 192 个基因上调,277 个基因下调,PPAR 信号通路在 KEGG 通路分析中排名第一。通过RT-qPCR分析和Western印迹,PPAR信号通路中的四个基因Fabp5、Acox2和Cpt2也在40 mg/kg组中得到了验证。我们的研究结果表明,长期注射甲苯噻嗪会导致非酒精性脂肪肝,而PPAR信号通路在甲苯噻嗪相关肝损伤过程中起着核心作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Repeated Injection of Xylazine Causes Liver Injury Through the PPAR Signaling Pathway in Rats

With the gradual emergence of xylazine as a street drug, incidents of xylazine poisoning are now occurring worldwide. However, it remains unknown whether long-term exposure to xylazine causes nonalcoholic fatty liver disease (NAFLD). In the present study, the rats were injected with xylazine intraperitoneally for 28 consecutive days, and then serum and liver tissues were collected for analysis. Weight loss was observed in the 40 mg/kg group and elevated levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were observed. Histopathologic examination showed hepatic steatosis, necrosis, and fibrosis. By mRNA sequencing, 192 upregulated genes and 277 downregulated genes were found in the 40 mg/kg group, and the PPAR signaling pathway was ranked first in the KEGG pathway analysis. Four genes in the PPAR signaling pathway, Fabp5, Acox2, and Cpt2, were also verified in the 40 mg/kg group by RT-qPCR analysis and western blot. Our results demonstrated that long-term injection of xylazine causes NAFLD and the PPAR signaling pathway plays a core role in the process of xylazine-associated liver injury.

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来源期刊
CiteScore
5.80
自引率
2.80%
发文量
277
审稿时长
6-12 weeks
期刊介绍: The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.
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