甲壳青霉四酸酯衍生物的生物合成揭示了邻甲醌参与多途径串扰

IF 5 1区化学 Q1 CHEMISTRY, ORGANIC

Organic Letters Pub Date : 2024-12-04 DOI:10.1021/acs.orglett.4c0369810.1021/acs.orglett.4c03698

Wen Li, Yu Dai, Hui-Ling Wei, Jenny Zhou, Jie Fan, Zhang-Hai Li, Xiulan Xie and Shu-Ming Li*,

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引用次数: 0

摘要

壳青霉的基因组挖掘和基因缺失实验证明PKS-NRPS PemA和反式烯醇还原酶PemB参与了三个对映体clavatoll四聚体对的形成。转录因子的过表达显著提高了产物的产量。饲养实验提供了证据，证明它们是通过1,4- michael将羟基clavatol添加到Pem途径的两种四聚体中形成的。这项研究提供了反应性中间体参与多种生物合成途径的另一个例子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Biosynthesis of Tetramate Derivatives in Penicillium crustosum Reveals the Involvement of ortho-Quinone Methide in Crosstalk of Multiple Pathways

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Biosynthesis of Tetramate Derivatives in Penicillium crustosum Reveals the Involvement of ortho-Quinone Methide in Crosstalk of Multiple Pathways

Genome mining and gene deletion experiments in Penicillium crustosum proved the involvement of the PKS-NRPS PemA and the trans-enoyl reductase PemB in the formation of three enantiomeric clavatol-containing tetramate pairs. Overexpression of a transcription factor significantly improved the product yields. Feeding experiments provided evidence for their formation via 1,4-Michael addition of hydroxyclavatol to two tetramates from the Pem pathway. This study provides another example of the involvement of reactive intermediates in multiple biosynthetic pathways.

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来源期刊

Organic Letters 化学-有机化学

CiteScore

9.30

自引率

11.50%

发文量

1607

审稿时长

1.5 months

期刊介绍： Organic Letters invites original reports of fundamental research in all branches of the theory and practice of organic, physical organic, organometallic,medicinal, and bioorganic chemistry. Organic Letters provides rapid disclosure of the key elements of significant studies that are of interest to a large portion of the organic community. In selecting manuscripts for publication, the Editors place emphasis on the originality, quality and wide interest of the work. Authors should provide enough background information to place the new disclosure in context and to justify the rapid publication format. Back-to-back Letters will be considered. Full details should be reserved for an Article, which should appear in due course.