细胞伸长和植物激素水平的改变在 Averrhoa carambola 的雌雄同株中起了作用。

IF 2.6 3区 生物学 Q2 GENETICS & HEREDITY
Gene Pub Date : 2024-12-14 DOI:10.1016/j.gene.2024.149167
Wubaiyu Lin, Si Qin, Siyu Chen, Lianhuan Xu, Zirui Yang, Xinyun Lin, Junwen Zhai, Hui Ren, Zehuang Zhang, Shasha Wu
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引用次数: 0

摘要

本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell elongation and altered phytohormone levels play a role in establishing distyly in Averrhoa carambola.

The flowers of distylous plants exhibit two distinct morphologies that facilitate precise pollen transfer. Averrhoa carambola, a woody plant characterized by distyly, has an unclear molecular regulatory mechanism underlying this trait. Its prolonged flowering period and substantial flower production render it an excellent model for investigating the distylous syndrome. This study aims to elucidate the mechanism of distyly in A. carambola and to identify the regulatory genes. The long-style cultivar 'Daguo Tianyangtao 1' and the short-style cultivar 'Daguo Tianyangtao 3' were selected as models for this investigation. We examined phenotypic characteristics, anatomical structures, and endogenous hormone content associated with distyly. Transcriptomic data were utilized to pinpoint candidate genes involved in the regulation of distyly, followed by a bioinformatics analysis these genes. The results indicate that variations in cell elongation contribute to the differential heights of stigmas and anthers in A. carambola, thereby resulting in the distylous syndrome. Auxins, Gibberellin A3 (GA3), Gibberellin A4 (GA4), and brassinolide (BL) were found to influence elongation of styles, whereas Gibberellin A1 (GA1) and GA4 affected filament elongation. Transcriptome sequencing analysis identified 34 hormone-related differentially expressed genes (DEGs) and 16 cell development-related DEGs in different morphs of pistils, and 29 hormone-related DEGs and 22 cell development-related DEGs were identified in different morphs of stamens. Four candidate genes-AcaBRU1, AcaPRE1, AcaXTH2, and AcaEXPA11-were found to possess conserved motifs characteristic of their respective families. Consequently, various plant hormones modulate the expression of response genes, leading to differences in elongation of style and filament cells between different flower types of A. carambola, thereby promoting the distylous syndrome. This study provides a theoretical basis for understanding the mechanisms of distyly formation in woody plants.

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来源期刊
Gene
Gene 生物-遗传学
CiteScore
6.10
自引率
2.90%
发文量
718
审稿时长
42 days
期刊介绍: Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.
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