R3HDM1变异与秦川牛生长和肉质性状的关系及其在脂质积累中的作用

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY

Gene Pub Date : 2024-12-14 DOI:10.1016/j.gene.2024.149177

Miaoli Wang, Wentao Zhang, Chuang Li, Chenyang Liu, Xiaoping He, Ziyi Zhang, Gong Cheng

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引用次数: 0

摘要

含 R3H 结构域的 1 (R3HDM1) 基因已成为影响剩余饲料摄入量和牛肉产量的候选基因。尽管如此，R3HDM1 的遗传变异及其对肉牛的影响仍未得到研究。本研究在秦川牛 R3HDM1 基因中发现了四个单核苷酸多态性（SNPs），其中 g.61695680 T > C SNP 与胸深和背膘厚显著相关。g.61695680 T > C 同义突变显著改变了 R3HDM1 的 RNA 二级结构和稳定性。RNA干扰实验表明，敲除R3HDM1可通过调节CEBPβ等关键致脂因子，减少牛前脂肪细胞的脂肪生成和脂质积累。

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Association of R3HDM1 variants with growth and meat quality traits in Qinchuan cattle and its role in lipid accumulation.

The R3H domain containing 1 (R3HDM1) gene has emerged as a candidate influencing residual feed intake and beef yield. Despite this, the genetic variation of R3HDM1 and its effects on beef cattle remain unexplored. This study identified four single nucleotide polymorphisms (SNPs) in the R3HDM1 gene of Qinchuan cattle, with the g.61695680 T > C SNP significantly associated with chest depth and backfat thickness. The g.61695680 T > C synonymous mutation significantly altered the RNA secondary structure and stability of R3HDM1. RNA interference experiments demonstrated that R3HDM1 knockdown reduced adipogenesis and lipid accumulation in bovine preadipocytes by modulating key adipogenic factors such as CEBPβ (P < 0.05), ACCα (P < 0.05), and ATGL (P < 0.01). These findings suggest that the g.61695680 T > C variants within R3HDM1 could serve as valuable molecular markers for selecting improved Qinchuan cattle, thus enhancing genetic selection strategies for beef production.

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.