Shiori Takeuchi, Shosei Imai, Takuya Terai, Robert E Campbell
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引用次数: 0
摘要
我们开发出了一种化学基因指示剂，其亲和力适用于哺乳动物细胞内钠离子（Na+）的成像。这种指示剂基于绿色荧光蛋白（GFP）和HaloTag的嵌合体，并用合成冠醚螯合剂标记，是通过合理设计和定向进化相结合产生的。在哺乳动物细胞中，当细胞用 20 mM Na+ 和离子螯合剂处理时，该指示剂的激发比增加了约 100%。
A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ions.
A chemigenetic indicator with an affinity suitable for imaging of intracellular sodium ions (Na+) in mammalian cells was developed. The indicator, based on a chimera of green fluorescent protein (GFP) and HaloTag labeled with a synthetic crown ether chelator, was produced by a combination of rational design and directed evolution. In mammalian cells the indicator exhibited an approximately 100% increase in excitation ratio when the cells were treated with 20 mM Na+ and an ionophore.
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