一对部分非互补肽核酸识别双链 DNA 中的错配位点。

IF 1.4 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Chemistry Letters Pub Date : 2024-12-10 eCollection Date: 2024-12-01 DOI:10.1093/chemle/upae234

Masanari Shibata, Osami Shoji, Yuichiro Aiba

{"title":"一对部分非互补肽核酸识别双链 DNA 中的错配位点。","authors":"Masanari Shibata, Osami Shoji, Yuichiro Aiba","doi":"10.1093/chemle/upae234","DOIUrl":null,"url":null,"abstract":"We have successfully achieved efficient recognition of mismatched sites in double-stranded DNA through the formation of an invasion complex by using partially noncomplementary peptide nucleic acids (PNAs). Owing to mismatches between 2 PNAs used for invasion, the undesired PNA/PNA duplex, which inhibits invasion complex formation, was destabilized. This approach overcame an inherent challenge in PNA invasion, in particular, undesired PNA/PNA duplex formation resulting from PNA complementarity, thereby enhancing overall invasion efficiency.","PeriodicalId":9862,"journal":{"name":"Chemistry Letters","volume":"53 12","pages":"upae234"},"PeriodicalIF":1.4000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11640769/pdf/","citationCount":"0","resultStr":"{\"title\":\"Recognition of mismatched sites in double-stranded DNA by a pair of partially noncomplementary peptide nucleic acids.\",\"authors\":\"Masanari Shibata, Osami Shoji, Yuichiro Aiba\",\"doi\":\"10.1093/chemle/upae234\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We have successfully achieved efficient recognition of mismatched sites in double-stranded DNA through the formation of an invasion complex by using partially noncomplementary peptide nucleic acids (PNAs). Owing to mismatches between 2 PNAs used for invasion, the undesired PNA/PNA duplex, which inhibits invasion complex formation, was destabilized. This approach overcame an inherent challenge in PNA invasion, in particular, undesired PNA/PNA duplex formation resulting from PNA complementarity, thereby enhancing overall invasion efficiency.\",\"PeriodicalId\":9862,\"journal\":{\"name\":\"Chemistry Letters\",\"volume\":\"53 12\",\"pages\":\"upae234\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-12-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11640769/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemistry Letters\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1093/chemle/upae234\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry Letters","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1093/chemle/upae234","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

我们利用部分非互补的肽核酸（PNA），通过形成入侵复合物，成功实现了对双链 DNA 中错配位点的高效识别。由于用于入侵的两个 PNA 之间存在错配，抑制入侵复合物形成的不想要的 PNA/PNA 双链失去了稳定性。这种方法克服了 PNA 侵染中固有的难题，特别是 PNA 互补性导致的 PNA/PNA 双链的形成，从而提高了整体侵染效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Recognition of mismatched sites in double-stranded DNA by a pair of partially noncomplementary peptide nucleic acids.

We have successfully achieved efficient recognition of mismatched sites in double-stranded DNA through the formation of an invasion complex by using partially noncomplementary peptide nucleic acids (PNAs). Owing to mismatches between 2 PNAs used for invasion, the undesired PNA/PNA duplex, which inhibits invasion complex formation, was destabilized. This approach overcame an inherent challenge in PNA invasion, in particular, undesired PNA/PNA duplex formation resulting from PNA complementarity, thereby enhancing overall invasion efficiency.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Chemistry Letters 化学-化学综合

CiteScore

3.00

自引率

6.20%

发文量

260

审稿时长

1.2 months

期刊介绍： Chemistry Letters covers the following topics: -Organic Chemistry- Physical Chemistry- Inorganic Chemistry- Analytical Chemistry- Materials Chemistry- Polymer Chemistry- Supramolecular Chemistry- Organometallic Chemistry- Coordination Chemistry- Biomolecular Chemistry- Natural Products and Medicinal Chemistry- Electrochemistry