通过调节糖酵解和抗坏血酸代谢,过表达 SlAN2 可提高番茄(Solanum lycopersicum L.)的抗寒性。

IF 3.4 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Minghui Ye, Deying Wang, Ruixin Li, Kunyang Zhuang, Hongjiao Wang, Xinyin Cao, Tengfei Qin, Hengjia Zhang, Shangjing Guo, Bingjie Wu
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引用次数: 0

摘要

寒冷胁迫严重影响番茄的生长和产量。花青素是一种典型的冷胁迫诱导代谢产物,具有很强的抗氧化活性和光保护能力。在这里,我们发现花青素还参与了冷胁迫下抗坏血酸的生物合成和糖酵解。SlAN2 是花青素生物合成过程中的一个重要阳性基因。生理指标结果表明,SlAN2过表达株系(A189)比野生型(WT)植株具有更强的耐寒胁迫能力。通过对A189株系和WT株系的转录组学和代谢组学的联合分析,分析了寒冷胁迫下花青素引起的代谢差异和抗寒机制。结果表明,在4 °C的寒冷胁迫下24 h,A189株系的花青素积累高于WT株系,导致己糖和抗坏血酸显著增加。结果表明,与野生品系相比,SlAN2 过表达会降低糖酵解途径中关键酶基因(如磷酸果糖激酶(PFK)和丙酮酸激酶(PK)基因)的表达量,削弱糖酵解能力,并促进 A189 品系在 4 ℃ 胁迫 24 小时后己糖的积累。此外,抗坏血酸过氧化物酶(APX)和脱氢抗坏血酸还原酶(DHAR)基因的上调也增加了抗坏血酸的含量。己糖含量的增加可降低细胞渗透势和冰点,合成更多的抗坏血酸,而抗坏血酸含量的增加可增强清除活性氧的能力,从而提高番茄的抗寒性。SlAN2 介导的糖酵解和抗坏血酸代谢途径为花青素提高番茄抗寒性的分子机制提供了新的认识,为培育耐寒番茄新品种提供了新的理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SlAN2 overexpression improves cold resistance in tomato (Solanum lycopersicum L.) by regulating glycolysis and ascorbic acid metabolism.

Chilling stress seriously affects the growth and yield of tomato. Anthocyanin is a typical chilling-induced metabolite with strong antioxidant activity and photoprotective capacity. Here, we found that anthocyanin was also involved in ascorbic acid biosynthesis and glycolysis under chilling stress. SlAN2 is an important positive gene in anthocyanin biosynthesis. The results of physiological indicators showed that SlAN2 overexpression lines (A189) had a greater ability to tolerate cold stress than wild-type (WT) plants. Conjoint analysis of transcriptomics and metabonomics of A189 lines and WT plants was used to analyze the metabolic difference and the cold resistance mechanisms caused by anthocyanin under chilling stress. The anthocyanin accumulated more in A189 than that in WT under chilling stress at 4 °C for 24 h, which led to hexoses and ascorbic acid increased significantly. Results indicate that SlAN2 overexpression reduces the expression of key enzyme genes in glycolytic pathway such as phosphofructokinase (PFK) and pyruvate kinase (PK) genes, weakens glycolysis ability, and promotes accumulation of hexoses in A189 lines at 4 °C for 24 h compared with wild lines. Additionally, ascorbic acid content is increased by up-regulated the genes of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR). The increased hexose content can reduce cell osmotic potential, freezing point and synthesize more ascorbic acid, while the increased ascorbic acid content can enhance the ability to scavenge reactive oxygen species, so improves the cold resistance of tomato. The glycolysis and ascorbic acid metabolism pathway mediated by SlAN2 provides a new insight for the molecular mechanism of anthocyanins in improving the cold resistance of tomato and provides a new theoretical basis for cultivating new cold-tolerant tomato varieties.

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来源期刊
Genomics
Genomics 生物-生物工程与应用微生物
CiteScore
9.60
自引率
2.30%
发文量
260
审稿时长
60 days
期刊介绍: Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.
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