烟雾病患者外周血单个核细胞的先天免疫细胞谱分析。

IF 4.5 2区 医学 Q2 CELL BIOLOGY
Chenglong Liu, Siqi Mou, Bojian Zhang, Yuheng Pang, Liujia Chan, Junsheng Li, Qiheng He, Zhiyao Zheng, Zhikang Zhao, Wei Sun, Xiangjun Shi, Hancheng Qiu, Xiaofeng Deng, Wenjing Wang, Peicong Ge, Jizong Zhao
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引用次数: 0

摘要

烟雾病是一种罕见的脑血管疾病,其特征是颈内动脉狭窄或闭塞,从而导致缺血性和出血性中风。尽管遗传学研究已经确定无名指蛋白213 (RNF213)是一个易感基因,但低疾病外显率表明,继发性触发因素(如感染)可能引发疾病发作。本研究旨在通过细胞计数技术(CyTOF)表征烟雾病患者外周血单个核细胞(PBMCs)的先天免疫细胞谱。对来自10名烟雾病患者和10名健康对照者的血液样本进行了分析,重点分析了自然杀伤细胞(NK)、单核细胞和树突状细胞(dc)。结果显示烟雾病患者NK细胞和单核细胞亚群发生显著变化;具体来说,CD56dimCD16- NK03亚群减少,cd163高经典单核细胞增加,从而表明微生物防御受损和炎症加剧。此外,在DC亚群中观察到显著变化,包括CCR7+成熟DC增加,CD141+和CD1c+ DC减少。在大多数先天免疫细胞中观察到TLR/MyD88/NF-κB通路的过度激活,从而表明其在疾病进展中的潜在作用。这些发现为烟雾病的免疫功能障碍提供了新的见解,并突出了潜在的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Innate Immune Cell Profiling in Peripheral Blood Mononuclear Cells of Patients with Moyamoya Disease.

Moyamoya disease (MMD) is a rare cerebrovascular disease characterized by stenosis or occlusion of the internal carotid artery, thus leading to ischaemic and haemorrhagic strokes. Although genetic studies have identified ring finger protein 213 (RNF213) as a susceptibility gene, the low disease penetrance suggests that a secondary trigger, such as infection, may initiate disease onset. This study aimed to characterize the innate immune cell profile of peripheral blood mononuclear cells (PBMCs) of MMD patients via mass cytometry (CyTOF). Blood samples from 10 MMD patients and 10 healthy controls were analysed, with a focus on natural killer (NK) cells, monocytes, and dendritic cells (DCs). The results revealed significant changes in the NK and monocyte subpopulations in MMD patients; specifically, there was a decrease in the CD56dimCD16- NK03 subset and an increase in CD163high classical monocytes, thus indicating compromised microbial defences and heightened inflammation. Additionally, significant changes were observed in DC subpopulations, including an increase in CCR7+ mature DCs and a decrease in CD141+ and CD1c+ DCs. Overactivation of the TLR/MyD88/NF-κB pathway was observed in most innate immune cells, thus indicating its potential role in disease progression. These findings provide novel insights into immune dysfunction in MMD and highlight potential therapeutic targets.

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来源期刊
Inflammation
Inflammation 医学-免疫学
CiteScore
9.70
自引率
0.00%
发文量
168
审稿时长
3.0 months
期刊介绍: Inflammation publishes the latest international advances in experimental and clinical research on the physiology, biochemistry, cell biology, and pharmacology of inflammation. Contributions include full-length scientific reports, short definitive articles, and papers from meetings and symposia proceedings. The journal''s coverage includes acute and chronic inflammation; mediators of inflammation; mechanisms of tissue injury and cytotoxicity; pharmacology of inflammation; and clinical studies of inflammation and its modification.
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