IF 2.6 3区 生物学 Q2 GENETICS & HEREDITY
Gene Pub Date : 2024-12-10 DOI:10.1016/j.gene.2024.149150
Chao Zhang, Lingli Jiang, Jieyu Qian, Guo Yu, Hongsheng Qing, Li Li, Jianxin Fu
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引用次数: 0

摘要

基本螺旋-环-螺旋(bHLH)家族是一组重要的转录因子,参与植物的生长、发育和次生代谢过程。矮牵牛(矮牵牛杂交种)是一种广受喜爱和广泛栽培的花园花卉,品种繁多,其中一些品种散发出迷人的芳香,深受人们喜爱。牵牛花的芳香诱惑主要源于挥发性苯并类化合物/苯丙类化合物,它们是主要的花香化合物。但 bHLH 转录因子是否调控牵牛花花香化合物的合成尚不清楚。在本研究中,我们通过研究矮牵牛 bHLH 基因家族的 63 个成员,试图筛选出可能参与苯并类化合物/苯丙类化合物生物合成的 bHLH 潜在候选成员。通过系统进化分析,将 63 个矮牵牛 bHLH 蛋白分为 16 个亚群。几乎所有 bHLH 成员都含有碱性/螺旋-环-螺旋结构域。根据所报告的杂交矮牵牛'米切尔'的 RNA 测序数据,30 个组装序列被映射到 axillaris 的 bHLH 基因上。进一步的 qRT-PCR 分析表明,PhbHLH19 可能是苯并类化合物/苯丙类化合物生物合成过程中的推定候选成员。PhbHLH19 在花瓣瓣缘的表达水平较高,但在花蕾期的表达水平较低,当花开放时表达水平迅速上升。PhbHLH19 的表达量与 PhPAL2 的表达量呈显著正相关,酵母单杂交试验验证了 PhbHLH19 能与 PhPAL2 的启动子结合。此外,双荧光素酶试验证明了PhbHLH19对PhPAL2的转录激活作用。这些研究结果表明,PhbHLH19可能是通过激活PhPAL2表达来调控苯并类化合物/苯丙类化合物合成的候选基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide analysis of basic helix-loop-helix (bHLH) transcription factors in petunia and identification of the putative candidate member involved in floral volatile benzenoids/phenylpropanoids metabolism.

The basic helix-loop-helix (bHLH) family, a prominent group of transcription factors, is involved in plant growth, development, and secondary metabolic processes. Petunia (Petunia hybrida), a beloved and widely cultivated garden flower, boasts a diverse array of varieties, some of which exude a captivating fragrance that has garnered immense popularity. The aromatic allure of petunias primarily stems from the presence of volatile benzenoids/phenylpropanoids, the principal floral scent compounds. But whether bHLH transcription factors regulate petunia floral scent compound synthesis is not clear. In this study, we sought to screen the putative candidate member of bHLH which can be involved in the biosynthesis of benzenoids/phenylpropanoids by examining 63 members of the petunia bHLH gene family. Phylogenetic analysis of the 63 petunia bHLH proteins them into 16 subgroups. Almost all bHLH members contained alkaline/helix-loop-helix domains. Based on the reported RNA sequencing data of P. hybrida 'Mitchell', 30 assembled sequences were mapped to the bHLH genes of P. axillaris. Further qRT-PCR assays suggested that PhbHLH19 might be the putative candidate member in the biosynthesis of benzenoids/phenylpropanoids. PhbHLH19 showed higher expression levels in the petal limb but was lowly expressed at the bud stage, with a rapid increase in the expression level when flowers opened. The expression of PhbHLH19 displayed a significant positive correlation with that of PhPAL2, and the yeast one-hybrid assay verified that PhbHLH19 can bind to the promoter of PhPAL2. Moreover, a dual-luciferase assay proved the transcriptional activation of PhbHLH19 on PhPAL2. These findings suggested that PhbHLH19 might be a putative candidate in the regulation of benzenoid/phenylpropanoid synthesis by activating PhPAL2 expression.

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来源期刊
Gene
Gene 生物-遗传学
CiteScore
6.10
自引率
2.90%
发文量
718
审稿时长
42 days
期刊介绍: Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.
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