预后生物标志物MICAL2及其与胰腺腺癌增殖、迁移和免疫浸润的关系

IF 2 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Huachao Yang, Pingping Yu, Jianping Gong
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引用次数: 0

摘要

阐明MICAL2作为PAAD潜在的免疫治疗靶点和预测性生物标志物的关键功能。利用公共数据库研究MICAL2在泛癌中的表达,利用组织样本验证MICAL2在PAAD中的表达。应用ROC曲线和Kaplan-Meier曲线评价MICAL2对PAAD的诊断和预后意义。利用TIMER和TCGA数据库研究MICAL2与PAAD浸润性免疫细胞和免疫检查点的相关性。在体外研究中,通过shRNA敲低MICAL2的表达来评估MICAL2在人PAAD细胞中的生物学功能。与相应的正常组织相比,MICAL2在各种癌症中的表达存在显著差异。具体来说,MICAL2的表达水平在PAAD中显著升高。此外,MICAL2在PAAD患者中显示出相当大的诊断潜力,其表达升高预示着不良预后。MICAL2在PAAD中的差异表达与浸润免疫细胞、免疫细胞标志物和免疫检查点有关。在ASPC-1和PANC-1细胞中,MICAL2被敲除后,其增殖、迁移和侵袭均受到明显抑制。MICAL2是PAAD预后评估的重要预测因子和有希望的免疫治疗靶点。它在促进PAAD细胞的生长和迁移中具有关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prognostic biomarker MICAL2 and associates with proliferation, migration and immune infiltration in pancreatic adenocarcinoma.

To elucidate the crucial function of MICAL2 as a potential immunotherapeutic target and a predictive biomarker in PAAD. The expression of MICAL2 in pan-cancer was investigated using public database, and the expression of MICAL2 in PAAD was validated using tissue samples. The diagnostic and prognostic significance of MICAL2 in PAAD was assessed through the application of ROC curves and Kaplan-Meier curves. The correlation between MICAL2 and infiltrating immune cells and immune checkpoints in PAAD was researched using the TIMER and TCGA databases. In vitro studies involved the evaluation of the biological functions of MICAL2 in human PAAD cells through the knockdown of MICAL2 expression using shRNA. Compared to corresponding normal tissues, the expression of MICAL2 exhibits significant differences in various cancers. Specifically, the level of MICAL2 expression is significantly increased in PAAD. Moreover, MICAL2 demonstrates considerable diagnostic potential in PAAD patients, and its elevated expression is indicative of an unfavorable prognosis. The differential expression of MICAL2 is related to infiltrating immune cells, immune cell markers, and immune checkpoints in PAAD. In ASPC-1 and PANC-1 cells, when MICAL2 was knocked down, there was a notable suppression of proliferation, migration, and invasion. MICAL2 functions as a significant predictor and promising immunotherapeutic target for prognosis assessment in PAAD. It has a pivotal function in fostering the growth and migration of PAAD cells.

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来源期刊
Journal of Applied Genetics
Journal of Applied Genetics 生物-生物工程与应用微生物
CiteScore
4.30
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: The Journal of Applied Genetics is an international journal on genetics and genomics. It publishes peer-reviewed original papers, short communications (including case reports) and review articles focused on the research of applicative aspects of plant, human, animal and microbial genetics and genomics.
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