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IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2024-12-09 DOI:10.1016/j.jep.2024.119226

Widad Dahmani, Zachée Louis Evariste Akissi, Nabia Elaouni, Nour Elhouda Bouanani, Hassane Mekhfi, Mohamed Bnouham, Abdelkhaleq Legssyer, Sevser Sahpaz, Abderrahim Ziyyat

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引用次数: 0

摘要

民族药理学意义：Ceratonia siliqua L. 是一种具有重要营养价值和工业价值的物种，具有广泛的传统用途。这种芸香科植物以其药用特性而闻名，包括治疗高血压。由于其化学成分，角豆树具有多种宝贵的治疗功能，如抗氧化、止泻、抗糖尿病和抗菌作用：本研究调查了 Ceratonia siliqua L. 叶水提取物（CsAE）的化学成分，并探讨了其血管舒张作用及其内在机制。此外，还研究了 CsAE 的急性毒性和抗氧化活性：方法：使用 TLC 和 UHPLC-MS 分析植物化学成分。使用各种拮抗剂对正常血压大鼠的胸主动脉环进行了血管舒张效应和机制研究。通过给小鼠口服提取物评估了急性毒性。使用β-胡萝卜素漂白和 DPPH 对抗氧化活性进行了评估：结果：CsAE 的 TLC 分析显示出黄酮类化合物和可水解单宁酸。通过超高效液相色谱-质谱（UHPLC-MS）鉴定出了没食子酸、没食子苷、槲皮苷以及五倍子酰吡喃葡萄糖苷衍生物。CsAE 能以浓度依赖的方式舒张肾上腺素预收缩的主动脉。当主动脉被剥离或用 L-NAME、羟钴胺、ODQ、4-AP、TEA、氯化苄达唑铵和硫司加精预处理时，这种反应会减弱。结论：CsAE 具有显著的抗氧化活性，对实验动物没有毒性：结论：CsAE 通过 CaM/eNOS/sGC 通路、Kca 和 Kv 的活化以及细胞内钙向 SERCA 的动员，具有明显的血管扩张作用。它还具有很强的抗氧化活性，在实验动物体内未观察到毒性。这些发现首次证明了摩洛哥东部的 Ceratonia siliqua L. 叶子具有舒张血管的作用。

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Carob leaves: Phytochemistry, antioxidant properties, vasorelaxant effect and mechanism of action.

Ethnopharmacological relevance: Ceratonia siliqua L., is a species of significant nutritional and industrial interest with extensive traditional uses. This fabaceae is renowned for its medicinal properties, including the treatment of high blood pressure. Due to its chemical composition, carob exhibits several valuable therapeutic functions such as antioxidant, antidiarrheal, antidiabetic, and antibacterial actions.

Aim of the review: This study investigates the chemical composition of Ceratonia siliqua L. leaves aqueous extract (CsAE) and explores the vasorelaxant effect and its underlying mechanisms. Acute toxicity and antioxidant activity of CsAE were also examined.

Methods: The phytochemical profile was elucidated using TLC and UHPLC-MS. The vasorelaxant effect and mechanisms were studied on thoracic aortic rings from normotensive rats, using various antagonists. Acute toxicity was assessed by orally administering the extract to mice. Antioxidant activity was evaluated using β-carotene bleaching and DPPH.

Results: TLC analysis of CsAE reveals flavonoids and hydrolysable tannins. Gallic acid, myricitrin, quercitrin as well as galloylglucopyranoside derivatives were identified by UHPLC-MS. CsAE relaxed phenylephrine-precontracted aorta in a concentration-dependent manner. This response was reduced when the aorta was denuded or pretreated with L-NAME, hydroxocobalamin, ODQ, 4-AP, TEA, calmidazolium chloride, and thapsigargin. CsAE showed significant antioxidant activity with no observed toxicity in the experimental animals.

Conclusion: CsAE has a significant vasodilatory effect, mediated through the CaM/eNOS/sGC pathway, activation of Kc_a and K_v, and intracellular calcium mobilization into SERCA. It also exhibits strong antioxidant activity, with no observed toxicity in the experimental animals. These findings represent the first evidence of the vasorelaxant effect of Ceratonia siliqua L. leaves from Eastern Morocco.

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.