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IF 3.2 3区医学

Journal of Diabetes Investigation Pub Date : 2024-12-09 DOI:10.1111/jdi.14369

Rafal Sibiak, Pawel Gutaj, Urszula Mantaj, Lukasz Adamczak, Malgorzata Blatkiewicz, Marcin Rucinski, Ewa Wender-Ozegowska

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引用次数: 0

摘要

目的/简介：胎盘葡萄糖转运受葡萄糖转运蛋白（GLUTs）调控。本研究旨在检测 1 型糖尿病患者、早期妊娠糖尿病（eGDM）患者和健康对照组的胎盘 GLUT-1、GLUT-3 和 GLUT-4 mRNA 的表达，并研究 GLUTs 表达与临床参数之间的相关性。此外，我们还比较了所招募亚组的胎盘转录组概况：我们招募了 59 名孕妇：23 名 1 型糖尿病患者、17 名 eGDM 患者和 19 名对照组。糖尿病患者参加了每个孕期的随访。对每个亚组的 4 名患者进行了转录组研究：所有亚组的平均年龄相似。eGDM 患者的体重指数（BMI）明显较高，且以肥胖为主。我们观察到 1 型糖尿病组和 eGDM 组的胎盘 GLUT-3 mRNA 表达量明显下降了 2 倍（P = 0.009）。eGDM 组的 GLUT-4 mRNA 表达明显低于 1 型糖尿病组（3 倍）和对照组（6 倍）（P = 0.007）。GLUT-3 (R = -0.29) 和 GLUT-4 (R = -0.27)mRNA表达与新生儿出生体重呈明显负相关。在 eGDM 患者中，GLUT-4 的表达与怀孕头三个月的 HbA1c（R = -0.72）和 OGTT 120'（R = -0.82）结果呈负相关；在 1 型糖尿病患者中，GLUT-4 的表达与怀孕第三个月的血糖变异性（R = -0.49）呈负相关。微阵列分析表明转录组发生了重大变化，1 型糖尿病患者有 45 个基因下调，365 个基因上调，而 eGDM 患者有 21 个基因发生了重大变化：结论：糖尿病患者的胎盘样本显示出 GLUTs 表达的变化，这与新生儿的生长和多个血糖参数相关。结论：糖尿病患者的胎盘样本显示出 GLUTs 表达的变化，这种变化与新生儿的生长和几种血糖参数相关。此外，在高血糖患者中还观察到转录组特征的多种变化。

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Placental expression of GLUT-1, GLUT-3, and GLUT-4 mRNA and transcriptome profiling in pregnant women with diabetes.

Aims/introduction: Placental glucose transport is regulated by glucose transporter proteins (GLUTs). The study aimed to examine placental expression of GLUT-1, GLUT-3, and GLUT-4 mRNA in patients with type 1 diabetes, early gestational diabetes (eGDM), and healthy controls, and to investigate correlations between GLUTs expression and clinical parameters. Additionally, we compared placental transcriptome profiles in recruited subgroups.

Materials and methods: We recruited 59 pregnant women: 23 with type 1 diabetes, 17 with eGDM, and 19 controls. Patients with diabetes attended follow-up visits at each trimester. Transcriptome studies were performed in 4 patients per subgroup.

Results: The mean age was similar across all subgroups. eGDM patients had significantly higher BMI and were predominantly obese. We observed a significant 2-fold (P = 0.009) decrease in placental GLUT-3 mRNA expression in the type 1 diabetes and eGDM groups. GLUT-4 mRNA expression was significantly lower in the eGDM group compared to type 1 diabetes (3-fold) and controls (6-fold) (P = 0.007). There was a significant negative correlation between GLUT-3 (R = -0.29) and GLUT-4 (R = -0.27) mRNA expression and neonatal birth weight. GLUT-4 expression was negatively correlated with 1st trimester HbA1c (R = -0.72) and OGTT 120' (R = -0.82) results in eGDM patients, and 3rd trimester glycemic variability (R = -0.49) in type 1 diabetes. Microarray analysis revealed significant transcriptomic changes, with 45 down-regulated and 365 up-regulated genes in type 1 diabetes, and 21 significant changes in eGDM.

Conclusions: Placental samples from patients with diabetes exhibit changes in GLUTs expression, which correlates with neonatal growth and several glycemic parameters. Additionally, multiple changes in transcriptomic profiles are observed in hyperglycemic patients.

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来源期刊

Journal of Diabetes Investigation Medicine-Internal Medicine

自引率

9.40%

发文量

218

期刊介绍： Journal of Diabetes Investigation is your core diabetes journal from Asia; the official journal of the Asian Association for the Study of Diabetes (AASD). The journal publishes original research, country reports, commentaries, reviews, mini-reviews, case reports, letters, as well as editorials and news. Embracing clinical and experimental research in diabetes and related areas, the Journal of Diabetes Investigation includes aspects of prevention, treatment, as well as molecular aspects and pathophysiology. Translational research focused on the exchange of ideas between clinicians and researchers is also welcome. Journal of Diabetes Investigation is indexed by Science Citation Index Expanded (SCIE).