貉ACE2与SARS-CoV-2原型及其变异的受体结合及结构基础

IF 5.5 1区医学 Q1 MICROBIOLOGY

PLoS Pathogens Pub Date : 2024-12-05 eCollection Date: 2024-12-01 DOI:10.1371/journal.ppat.1012713

Chunliang Luo, Linjie Li, Yuhang Gu, Hangchuan Zhang, Zepeng Xu, Junqing Sun, Kaiyuan Shi, Sufang Ma, Wen-Xia Tian, Kefang Liu, George F Gao

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引用次数: 0

摘要

浣熊被认为是SARS-CoV-2的潜在宿主，但没有证据支持这一观点。在本研究中，我们研究了貉ACE2 （rdACE2）与SARS-CoV-2原型病毒（PT）及其变体的spike (S)蛋白受体结合域（RBD）的结合亲和力。结果表明，来自SARS-CoV-2变体的RBD的结合亲和力普遍低于PT RBD。通过结构和功能分析，我们发现氨基酸H34和M82在维持ACE2与不同SARS-CoV-2亚变体的结合亲和力方面发挥了关键作用。这些结果表明，与SARS-CoV-2传播率高的动物物种相比，貉对SARS-CoV-2的易感性较低。

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Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants.

Raccoon dog was proposed as a potential host of SARS-CoV-2, but no evidence support such a notion. In our study, we investigated the binding affinities of raccoon dog ACE2 (rdACE2) to the spike (S) protein receptor binding domain (RBD) of SARS-CoV-2 prototype (PT) and its variants. It revealed that the binding affinities of RBD from SARS-CoV-2 variants were generally lower than that of the PT RBD. Through structural and functional analyses, we found amino acids H34 and M82 play pivotal roles in maintaining the binding affinity of ACE2 to different SARS-CoV-2 sub-variants. These results suggest that raccoon dogs exhibit lower susceptibility to SARS-CoV-2 compared to those animal species with a high prevalence of SARS-CoV-2 transmission.

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来源期刊

PLoS Pathogens MICROBIOLOGY-PARASITOLOGY

自引率

3.00%

发文量

598

期刊介绍： Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.