{"title":"基于香豆素的 Tb3+ 复合物的合成及其在细胞成像中的应用","authors":"Sneha Wankar, Sushma Bankar","doi":"10.1016/j.molstruc.2024.140777","DOIUrl":null,"url":null,"abstract":"<div><div>Mitochondria is an important site for ATP production from oxidative phosphorylation, and plays a crucial role in biochemical processes. It is a significant site for the production of ATP via oxidative phosphorylation, and plays crucial in regulation of apoptosis, redox homeostasis and calcium signalling. In the present work, Tb<sup>3+</sup> complex was synthesized using coumarin based ligand <strong>L</strong> and 1, 10-phenanthroline as coligand. The coumarin is well known for its photophysical and medicinal importance in therapeutic research. Photophysical characterizations of the complex indicated that the ligand efficiently sensitizes the emissive level of Tb<sup>3+</sup> ion. Thus, the long-excited state lifetime of 0.75 ms and high quantum yield of 57 % exhibited by the complex led to its employment in imaging of cellular organelles. Interestingly, the complex was found to be selectively localized to mitochondria, its positively charged chemical structure being permeable to the cell membrane and accumulating in mitochondria, which is well visualized under confocal microscopy. An attempt has been made to understand how the Tb<sup>3+</sup> complex can be taken up by mitochondria and become a better target for drugs that exhibit therapeutic effect.</div></div>","PeriodicalId":16414,"journal":{"name":"Journal of Molecular Structure","volume":"1324 ","pages":"Article 140777"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Synthesis of coumarin based Tb3+ complex and its application in cell imaging\",\"authors\":\"Sneha Wankar, Sushma Bankar\",\"doi\":\"10.1016/j.molstruc.2024.140777\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Mitochondria is an important site for ATP production from oxidative phosphorylation, and plays a crucial role in biochemical processes. It is a significant site for the production of ATP via oxidative phosphorylation, and plays crucial in regulation of apoptosis, redox homeostasis and calcium signalling. In the present work, Tb<sup>3+</sup> complex was synthesized using coumarin based ligand <strong>L</strong> and 1, 10-phenanthroline as coligand. The coumarin is well known for its photophysical and medicinal importance in therapeutic research. Photophysical characterizations of the complex indicated that the ligand efficiently sensitizes the emissive level of Tb<sup>3+</sup> ion. Thus, the long-excited state lifetime of 0.75 ms and high quantum yield of 57 % exhibited by the complex led to its employment in imaging of cellular organelles. Interestingly, the complex was found to be selectively localized to mitochondria, its positively charged chemical structure being permeable to the cell membrane and accumulating in mitochondria, which is well visualized under confocal microscopy. An attempt has been made to understand how the Tb<sup>3+</sup> complex can be taken up by mitochondria and become a better target for drugs that exhibit therapeutic effect.</div></div>\",\"PeriodicalId\":16414,\"journal\":{\"name\":\"Journal of Molecular Structure\",\"volume\":\"1324 \",\"pages\":\"Article 140777\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-11-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Molecular Structure\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S002228602403285X\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Structure","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S002228602403285X","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
摘要
线粒体是通过氧化磷酸化产生 ATP 的重要场所,在生化过程中发挥着至关重要的作用。线粒体是通过氧化磷酸化产生 ATP 的重要场所,在调节细胞凋亡、氧化还原平衡和钙信号传导中起着至关重要的作用。本研究使用香豆素配体 L 和 1,10-菲罗啉作为副配体合成了 Tb3+ 复合物。众所周知,香豆素在光物理和药物治疗研究方面具有重要意义。该复合物的光物理特性表明,配体能有效敏化 Tb3+ 离子的发射水平。因此,该复合物具有 0.75 毫秒的长激发态寿命和 57% 的高量子产率,可用于细胞器成像。有趣的是,研究发现该复合物可选择性地定位在线粒体中,其带正电荷的化学结构可渗透细胞膜,并在线粒体中聚集,这在共聚焦显微镜下可以很好地观察到。我们试图了解 Tb3+ 复合物如何被线粒体吸收,并成为具有治疗效果的药物的更好靶点。
Synthesis of coumarin based Tb3+ complex and its application in cell imaging
Mitochondria is an important site for ATP production from oxidative phosphorylation, and plays a crucial role in biochemical processes. It is a significant site for the production of ATP via oxidative phosphorylation, and plays crucial in regulation of apoptosis, redox homeostasis and calcium signalling. In the present work, Tb3+ complex was synthesized using coumarin based ligand L and 1, 10-phenanthroline as coligand. The coumarin is well known for its photophysical and medicinal importance in therapeutic research. Photophysical characterizations of the complex indicated that the ligand efficiently sensitizes the emissive level of Tb3+ ion. Thus, the long-excited state lifetime of 0.75 ms and high quantum yield of 57 % exhibited by the complex led to its employment in imaging of cellular organelles. Interestingly, the complex was found to be selectively localized to mitochondria, its positively charged chemical structure being permeable to the cell membrane and accumulating in mitochondria, which is well visualized under confocal microscopy. An attempt has been made to understand how the Tb3+ complex can be taken up by mitochondria and become a better target for drugs that exhibit therapeutic effect.
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