用于细胞毒性和传感应用的柠檬酸功能化新霉素碳点

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Structure Pub Date : 2024-11-16 DOI:10.1016/j.molstruc.2024.140769

Pritesh P. Khobrekar , Geeta A. Zalmi , Antara P. Raiturker , Ratan W. Jadhav , Anasuya Ganguly , Avelyno D'Costa , Sandesh T. Bugde , Sheshanath V. Bhosale

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引用次数: 0

摘要

碳纳米材料已被应用于各个领域。目前，具有实用调谐和表面功能化的功能化碳纳米材料是一种应用广泛的方法。本文以新霉素和柠檬酸为原料，通过水热法合成了新霉素基荧光碳量子点，用于选择性传感 Fe3+ 金属离子。制备的 Neo-CA-CQDs 发出蓝色荧光，并成功表征和用于传感各种金属离子。该碳点对 Fe3+ 金属离子具有极佳的传感性能，其光学特性通过荧光光谱仪进行了表征。此外，还对 Neo-CA-CQDs 的 pH 值和生物相容性进行了研究。Neo-CA-CQDs 的平均尺寸为 3-5.7 nm。制备的 Neo-CA-CQDs 的量子产率为 29.3%。测定的 Fe3+ 检测限为 6.09 μM。通过目视检测也证实了对 Fe3+ 离子的感应，因为观察到 CQDs 有明显的颜色变化。此外，还进行了竞争性研究，以验证柠檬酸新霉素介导的 CQDs 对 Fe3+ 的选择性。体外细胞毒性研究是在小鼠成纤维细胞系（L929）和人类宫颈癌细胞系（HeLa）上进行的。

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Citric acid functionalized neomycin carbon dots for cytotoxicity and sensing application

Carbon nanomaterials have been applied in various fields. Currently, functionalized carbon nanomaterial with practical tuning and surface functionalization is a tremendously used method. Herein, neomycin-based fluorescent carbon quantum dots were synthesized via hydrothermal method using neomycin and citric acid as a source for selective sensing of Fe³⁺ metal ions. The as-prepared Neo-CA-CQDs exhibited blue fluorescence, successfully characterized and utilized for sensing various metal ions. The carbon dots showed excellent sensing properties towards Fe³⁺ metal ions and optical properties were characterized by a fluorescence spectrofluorometer. In addition, pH and biocompatibility studies were investigated for the Neo-CA-CQDs. The average size of the Neo-CA-CQDs was 3–5.7 nm. The quantum yield of the as-prepared Neo-CA-CQDs was 29.3 %. The limit of detection for Fe³⁺ sensing was determined to be 6.09 μM. The sensing of Fe³⁺ ions was also confirmed through visual detection, as a significant color change of the CQDs was observed. Additionally, a competitive study was conducted to verify the selectivity of the neomycin citric acid-mediated CQDs towards Fe³⁺. The in vitro cytotoxicity study was performed on a mouse fibroblast cell line (L929) and Human cervical cancer cell line (HeLa).

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.