猪脱细胞神经基质水凝胶通过抑制 TLR4/MyD88/NF-κB 轴减轻周围神经损伤后的神经炎症。

IF 5.9 2区 医学 Q2 CELL BIOLOGY
Neural Regeneration Research Pub Date : 2026-03-01 Epub Date: 2024-11-13 DOI:10.4103/NRR.NRR-D-24-00302
Rui Li, Jianquan Liu, Liuxun Li, Guotian Luo, Xinrong Yuan, Shichao Shen, Yongpeng Shi, Jianlong Wu, Bin Yan, Lei Yang
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引用次数: 0

摘要

周围神经损伤会引起严重的神经炎症,已成为全球性的医学难题。以往的研究表明,猪脱细胞神经基质水凝胶具有优异的生物特性和组织特异性,突出了其作为修复严重周围神经损伤的生物医学材料的潜力;然而,它在周围神经损伤后调节神经炎症方面的作用仍不清楚。在此,我们旨在研究猪脱细胞神经基质水凝胶的抗炎特性及其潜在的分子机制。我们使用猪脱细胞神经基质水凝胶治疗周围神经损伤模型大鼠,评估了其体内的结构和功能恢复、巨噬细胞表型改变、特异性细胞因子表达以及相关信号分子的变化。在体外,我们使用单核细胞/巨噬细胞系在脂多糖的刺激下,在猪脱细胞神经基质水凝胶涂层板上用完全培养基进行培养,评估了类似的参数。这些综合分析表明,猪脱细胞神经基质水凝胶可减轻周围神经损伤早期的过度炎症激活,并增加单核细胞/巨噬细胞中 M2 亚型的比例。此外,猪脱细胞神经基质水凝胶还能在体内和体外负向调节Toll样受体4/髓系分化因子88/核因子-κB轴。我们的研究结果表明,猪脱细胞神经基质水凝胶的抗炎特性是通过抑制Toll样受体4/髓系分化因子88/核因子-κB通路诱导M2巨噬细胞极化,为猪脱细胞神经基质水凝胶在周围神经损伤中的治疗机制提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Porcine decellularized nerve matrix hydrogel attenuates neuroinflammation after peripheral nerve injury by inhibiting the TLR4/MyD88/NF-κB axis.

JOURNAL/nrgr/04.03/01300535-202603000-00045/figure1/v/2025-06-16T082406Z/r/image-tiff Peripheral nerve injury causes severe neuroinflammation and has become a global medical challenge. Previous research has demonstrated that porcine decellularized nerve matrix hydrogel exhibits excellent biological properties and tissue specificity, highlighting its potential as a biomedical material for the repair of severe peripheral nerve injury; however, its role in modulating neuroinflammation post-peripheral nerve injury remains unknown. Here, we aimed to characterize the anti-inflammatory properties of porcine decellularized nerve matrix hydrogel and their underlying molecular mechanisms. Using peripheral nerve injury model rats treated with porcine decellularized nerve matrix hydrogel, we evaluated structural and functional recovery, macrophage phenotype alteration, specific cytokine expression, and changes in related signaling molecules in vivo . Similar parameters were evaluated in vitro using monocyte/macrophage cell lines stimulated with lipopolysaccharide and cultured on porcine decellularized nerve matrix hydrogel-coated plates in complete medium. These comprehensive analyses revealed that porcine decellularized nerve matrix hydrogel attenuated the activation of excessive inflammation at the early stage of peripheral nerve injury and increased the proportion of the M2 subtype in monocytes/macrophages. Additionally, porcine decellularized nerve matrix hydrogel negatively regulated the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB axis both in vivo and in vitro . Our findings suggest that the efficacious anti-inflammatory properties of porcine decellularized nerve matrix hydrogel induce M2 macrophage polarization via suppression of the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway, providing new insights into the therapeutic mechanism of porcine decellularized nerve matrix hydrogel in peripheral nerve injury.

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来源期刊
Neural Regeneration Research
Neural Regeneration Research CELL BIOLOGY-NEUROSCIENCES
CiteScore
8.00
自引率
9.80%
发文量
515
审稿时长
1.0 months
期刊介绍: Neural Regeneration Research (NRR) is the Open Access journal specializing in neural regeneration and indexed by SCI-E and PubMed. The journal is committed to publishing articles on basic pathobiology of injury, repair and protection to the nervous system, while considering preclinical and clinical trials targeted at improving traumatically injuried patients and patients with neurodegenerative diseases.
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