来自不同免疫库的抗体数据分析

Cold Spring Harbor protocols Pub Date : 2024-11-25 DOI:10.1101/pdb.prot108627

Ahmed S Fahad, Matías F Gutiérrez-Gonzalez, Bharat Madan, Brandon J DeKosky

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引用次数: 0

摘要

抗体功能筛选研究和新一代测序需要对序列数据进行仔细处理和解读，以获得最佳结果。在此，我们提供了抗体基因数据功能分析的详细方案，包括基于富集比值的抗体谱系定量和高通量筛选数据的功能图谱，富集比值是针对所需抗原对展示文库进行分选后确定每个测序抗体富集度的一种简单方法。该方案能让用户应用一套简单而强大的生物信息学工具，对抗体数据进行高通量分析和解读，尤其适合展示文库筛选和抗体发现应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Antibody Data Analysis from Diverse Immune Libraries.

Antibody functional screening studies and next-generation sequencing require careful processing and interpretation of sequence data for optimal results. Here, we provide a detailed protocol for the functional analysis of antibody gene data, including antibody repertoire quantification and functional mapping of high-throughput screening data based on enrichment ratio values, which are a simple way to determine the enrichment of each sequenced antibody after sorting a display library against desired antigens. This protocol enables a user to apply a set of simple yet powerful bioinformatic tools for high-throughput analysis and interpretation of antibody data that is especially well suited for display library screening and for antibody discovery applications.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.