研究复杂样本中的结构细节:II.高场不对称波形离子迁移谱(FAIMS)与高分辨率串联质谱(MS/MS)联用。

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Alessandro Vetere, Wolfgang Schrader
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引用次数: 0

摘要

在极其复杂的混合物中阐明结构模式是非常困难的,因为标准的结构阐明方法无法提供有关单个分子的重要信息。分析复杂混合物的最佳方法是超高分辨率质谱法,但单独使用这种方法并不能提供有关结构细节的重要信息。在这种情况下,必须结合分离方法。虽然色谱法是一种成熟的技术,但它在分离复杂混合物方面有一些缺点,因为往往无法分离出单个异构体。因此,这里对离子迁移率分离法与超高分辨率质谱法的结合进行了评估。使用原油作为样品基质是因为它是开发复杂样品新分析技术的绝佳基质。原油是已知最复杂的天然样品,但由于存在大量结构异构体或异构体,因此有关其结构特性或功能的信息很少。对实验室自建的 APPI/APLI-FAIMS 源进行了改进,以优化离子传输,并用于跟踪原油成分在光离子化后的离子迁移率。利用碰撞诱导解离 (CID) 的 MS/MS 方法阐明了透射异构体的结构模式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studying Structural Details in Complex Samples: II. High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Coupled to High Resolution Tandem Mass Spectrometry (MS/MS).

The elucidation of structural motifs in extremely complex mixtures is very difficult since the standard methods for structural elucidation are not capable to provide significant information on a single molecule. The best method for the analysis of complex mixtures is ultrahigh resolution mass spectrometry, but the utilization of this method alone does not provide significant information about structural details. Here, a combination with a separation method is necessary. While chromatography is a well-established technique, it has some disadvantages in regard to the separation of complex mixtures, as often no separation of individual isomers is possible. Therefore, here the combination of an ion mobility separation with ultrahigh resolution mass spectrometry is evaluated. As a sample matrix, crude oil is used because it is an excellent matrix to develop new analytical techniques on complex samples. Crude oil is the most complex natural sample known, but only little information is available on the structural identity or functionalities due to a high number of structural isomers or isobars. A lab-built APPI/APLI-FAIMS source was revised to optimize ion transmission and used to follow up on the ion mobility of crude oil constituents after photoionization. An MS/MS approach using collision-induced dissociation (CID) was used to elucidate structural motifs of the transmitted isomers.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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