福氏内皮性角膜营养不良症患者白内障手术后与炎症相关的水液分子变化

IF 2.6 3区医学 Q2 OPHTHALMOLOGY

Ophthalmology and Therapy Pub Date : 2024-11-24 DOI:10.1007/s40123-024-01072-0

Lizaveta Chychko, Hyeck-Soo Son, Maximilian Friedrich, Ramin Khoramnia, Gerd U Auffarth, Victor A Augustin

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引用次数: 0

摘要

引言目的：通过量化福氏内皮角膜营养不良症（FECD）患者房水中的氧化应激和炎症介质，评估白内障手术后早期和戴斯麦膜内皮角膜移植术（DMEK）前的前房炎症情况：在这项前瞻性单中心研究中，15 名 FECD 患者分两步接受了白内障手术和 DMEK。在白内障手术开始时和 3 个月后 DMEK 手术开始时，分别从白内障房水中收集房水。对照组由 15 名年龄匹配、无 FECD 的晶状体患者组成，仅在白内障手术开始时收集房水。术后炎症介质包括 TNF-α、VEGF、IL-2、IL-1 β、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12、GM-CSF、IFN-γ、CXCL5/ENA-78、FGF-basic、G-CSF、使用多重阵列系统检测了 IL-1-α、IL-1-ra、IL-17、CCL2/MCP-1、CCL3/MIP-1a、CCL4/MIP-1b、TPO、TGF-β-1、TGF-β-2 和 TGF-β-3 的浓度。结果显示与白内障手术开始时的对照组相比，白内障手术开始 3 个月后 DMEK 组的 TNF-α (p = 0.021)、IL-6 (p = 0.005)、IL-8 (p = 0.001)、CXCL5/ENA78 (p = 0.002)、CCL2/MCP-1 (p = 0.001) 和 CCL4/MIP-1b (p = 0.037) 的浓度明显升高。在对照组中，IL-2、IL-5、IL-8、IL-10 和 IL-1-α 的水平在包膜眼中明显升高（p 结论：IL-2、IL-5、IL-8、IL-10 和 IL-1-α 的水平在包膜眼中明显升高：本研究表明，白内障手术后 3 个月，FECD 患眼的促炎细胞因子水平明显升高。我们的研究结果表明，在白内障手术后的 3 个月内，白内障角膜中都存在术后炎症。因此，对患有 FECD 的白内障患者来说，将白内障手术与 DMEK 结合使用可能是合理的。

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Molecular Changes in Aqueous Humor Associated with Inflammation Following Cataract Surgery in Patients with Fuchs' Endothelial Corneal Dystrophy.

Introduction: To evaluate the anterior chamber (AC) inflammation in the early postoperative period after cataract surgery and before Descemet membrane endothelial keratoplasty (DMEK) by quantifying oxidative stress and inflammatory mediators in aqueous humor of patients with Fuchs' endothelial corneal dystrophy (FECD).

Methods: In this prospective single-center study, 15 patients with FECD underwent cataract surgery and DMEK in a two-stage procedure. Aqueous humor was collected from the AC at the beginning of cataract surgery and 3 months later at the beginning of DMEK. In the control group, which consisted of 15 age-matched phakic patients without FECD, aqueous humor was only collected at the beginning of cataract surgery. Mediators of postoperative inflammation including TNF-α, VEGF, IL-2, IL-1 β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, GM-CSF, IFN-γ, CXCL5/ENA-78, FGF-basic, G-CSF, IL-1-α, IL-1-ra, IL-17, CCL2/MCP-1, CCL3/MIP-1a, CCL4/MIP-1b, TPO, TGF-β-1, TGF-β-2, and TGF-β-3 concentrations were measured using a Multiplex-Array-System.

Results: The concentration of TNF-α (p = 0.021), IL-6 (p = 0.005), IL-8 (p = 0.001), CXCL5/ENA78 (p = 0.002), CCL2/MCP-1 (p = 0.001) and CCL4/MIP-1b (p = 0.037) were significantly higher 3 months after cataract surgery at the beginning of DMEK compared to control group at beginning of cataract surgery. The levels of IL-2, IL-5, IL-8, IL-10, and IL-1-α were significantly higher in phakic eyes in the control group (p < 0.05) before cataract surgery.

Conclusions: The present study indicates significantly increased proinflammatory cytokines 3 months after cataract surgery in eyes with FECD. Our findings suggest postoperative inflammation in the AC up to 3 months after cataract surgery. Therefore, it may be reasonable to combine cataract surgery with DMEK in cataract patients with FECD.

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来源期刊

Ophthalmology and Therapy OPHTHALMOLOGY-

CiteScore

4.20

自引率

3.00%

发文量

157

审稿时长

6 weeks

期刊介绍： Aims and Scope Ophthalmology and Therapy is an international, open access, peer-reviewed (single-blind), and rapid publication journal. The scope of the journal is broad and will consider all scientifically sound research from preclinical, clinical (all phases), observational, real-world, and health outcomes research around the use of ophthalmological therapies, devices, and surgical techniques. The journal is of interest to a broad audience of pharmaceutical and healthcare professionals and publishes original research, reviews, case reports/series, trial protocols and short communications such as commentaries and editorials. Ophthalmology and Therapy will consider all scientifically sound research be it positive, confirmatory or negative data. Submissions are welcomed whether they relate to an international and/or a country-specific audience, something that is crucially important when researchers are trying to target more specific patient populations. This inclusive approach allows the journal to assist in the dissemination of quality research, which may be considered of insufficient interest by other journals. Rapid Publication The journal’s publication timelines aim for a rapid peer review of 2 weeks. If an article is accepted it will be published 3–4 weeks from acceptance. The rapid timelines are achieved through the combination of a dedicated in-house editorial team, who manage article workflow, and an extensive Editorial and Advisory Board who assist with peer review. This allows the journal to support the rapid dissemination of research, whilst still providing robust peer review. Combined with the journal’s open access model this allows for the rapid, efficient communication of the latest research and reviews, fostering the advancement of ophthalmic therapies. Open Access All articles published by Ophthalmology and Therapy are open access. 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