广藿香精油通过JNK-1/DAF-16延长秀丽隐杆线虫的寿命和健康期

IF 5.2 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Life sciences Pub Date : 2024-11-23 DOI:10.1016/j.lfs.2024.123270

Rui Xu , Ai-Pei Li , Xin Tan , Xue Tang , Xiao-Ping He , Li-Xia Wang , Juan-Juan Kang , Sheng-Hong Li , Yan Liu

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引用次数: 0

摘要

目的：广藿香精油（PEO）是一种著名的药用植物广藿香（Pogostemon cablin (Blanco) Benth.）的主要活性成分。本研究旨在探讨 PEO 及其主要成分的抗衰老活性，并阐明其分子机制：主要方法：通过检测秀丽隐杆线虫的寿命、衰老相关指标以及抗应激能力，研究 PEO 及其主要成分广藿香醇（PA）的抗衰老活性。采用 RNA 序列分析法分析了经 PEO 处理的线虫体内的差异表达基因以及 GO 和 KEGG 通路的富集情况。利用网络药理学方法和分子对接预测了潜在的抗衰老靶点。利用线虫突变体和GFP转基因株探讨了延缓衰老作用的内在机制：主要发现：PEO调节了蠕虫的寿命和健康期的延长，改善了衰老特征，并提高了抗应激试验中的存活率。PEO 降低了线虫的产卵、脂质积累和活性氧（ROS）水平。经 PEO 处理后，抗氧化基因和蛋白质的水平明显上调。此外，还发现 PA 是 PEO 介导线虫长寿的一个成分。JNK-1/DAF-16信号通路在PEO/PA介导的长寿过程中发挥了关键作用：研究结果表明，PEO及其主要成分PA通过调节JNK-1/DAF-16信号通路具有显著的抗衰老活性，为治疗衰老和老年相关疾病提供了一种有前景的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Patchouli essential oil extends the lifespan and healthspan of Caenorhabditis elegans through JNK-1/DAF-16

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Patchouli essential oil extends the lifespan and healthspan of Caenorhabditis elegans through JNK-1/DAF-16

Aims

Patchouli essential oil (PEO) is the major active ingredient of a famous medicinal plant Pogostemon cablin (Blanco) Benth. This study aims to investigate the anti-aging activities of PEO and its major component, and elucidate the underlying molecular mechanisms.

Main methods

The anti-aging activities of PEO and its main component patchouli alcohol (PA) were investigated by examining the lifespan, senescence associated indicators as well as stress resistance of Caenorhabditis elegans. RNA-Sequencing was performed to analyze differentially expressed genes and the enrichments of GO and KEGG pathways in nematodes treated with PEO. The potential anti-aging target was predicted using a network pharmacology method and molecular docking. The underlying mechanism of senescence-delaying action was explored using C. elegans mutants and GFP transgenic strains.

Key findings

PEO modulated lifespan and healthspan extension of worms, ameliorated the senescence characterizations, and increased the survival in stress resistance assays. PEO reduced spawning, lipid accumulation and reactive oxygen species (ROS) levels of nematodes. The levels of anti-oxidative genes and proteins were obviously upregulated after PEO treatment. Moreover, PA was identified to be an ingredient for PEO-mediated nematode longevity. The JNK-1/DAF-16 signaling pathway played a critical role in PEO/PA-mediated longevity.

Significance

The findings revealed that PEO and its major component PA showed significant anti-aging activity through modulating the JNK-1/DAF-16 signaling pathway, which provides a promising strategy to treat aging and age-related diseases.

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来源期刊

Life sciences 医学-药学

CiteScore

12.20

自引率

1.60%

发文量

841

审稿时长

6 months

期刊介绍： Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed. The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.