筛选蕉叶中用于烯烃化学酶促环氧化的脂肪酶 TiL

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2024-11-22 DOI:10.1016/j.enzmictec.2024.110547

Jiang Pan , Nan Yang , Yuan-Lin Lv , Zi-Yang Zhang , Chun-Xiu Li, Jian-He Xu

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引用次数: 0

摘要

脂肪酶可以在存在游离羧酸和过氧化氢的情况下介导烯烃的化学酶促环氧化反应。我们从基因数据库中挖掘出四种具有化学酶促环氧化能力的新型脂肪酶。确定了正庚酸为 TiL 的最佳羧酸底物。在微水介质和水有机双相中，油酸甲酯和 α-蒎烯分别被高效地转化为相应的环氧化合物。利用优化的反应条件对α-蒎烯进行了制备规模的化学酶转化，获得了 30% 的α-蒎烯氧化物收率。

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Screening of lipase TiL from Tilletia indica for chemo-enzymatic epoxidation of alkenes

Lipase can mediate the chemo-enzymatic epoxidation of alkenes with the presence of free carboxylic acid and hydrogen peroxide. Four novel lipases with the abilities of chemo-enzymatic epoxidation were mined from the gene database. Lipase TiL originated from Tilletia indica was identified with significant activity on formation of methyl epoxystearate from methyl oleate. n-Heptanoic acid was determined as the optimal carboxylic acid substrate of TiL. Methyl oleate and α-pinene were efficiently converted to corresponding epoxy compound in micro-aqueous media and aqueous-organic biphase, respectively. A preparative scale chemo-enzymatic transformation of α-pinene was conduct using the optimized reaction condition, with 30 % yield of α-pinene oxide obtained.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.