Bergenia ciliata (Haw.) Sternb.花提取物和生物活性化合物通过细胞膜损伤对变异链球菌的抗菌和抗生物膜活性。

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2024-11-21 DOI:10.1016/j.jep.2024.119144

Nirza Moktan , Rahul Laxman Gajbhiye , T.V.V.S. Sahithi , Dijendra Nath Roy , Rita Kundu , Anindita Banerjee

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引用次数: 0

摘要

民族药理学意义：Bergenia ciliata (Haw.) Sternb.（Saxifragaceae 科）在阿育吠陀中被称为 Pashanbheda，在尤那尼中被称为 Zakhmehayat。在巴基斯坦北瓦济里斯坦，土著社区在民族牙科中使用这种植物治疗蛀牙和牙痛。然而，有关其作用模式的科学证据仍然缺乏：研究目的：评估纤毛虫花提取物和馏分对口腔细菌的作用，并阐明可能的抗菌和抗生物膜机制：检查制备的纤毛虫花提取物对口腔细菌（变异性嗜血杆菌、化脓性嗜血杆菌、口腔嗜血杆菌）和机会性细菌（金黄色葡萄球菌、克罗恩柠檬酸杆菌和破产阿奇霉素）的抗菌活性。采用制备型 TLC 生物层析和硅胶柱层析分离生物活性化合物。利用 HRESI-MS 和 NMR 研究对其结构进行了阐明。研究了提取物和分离出的化合物对 S. mutans 的抗菌和抗生物膜活性。扫描电子显微镜研究表明存在膜损伤。此外，还评估了活性氧生成（ROS）、脂质过氧化和细胞质渗漏：结果：活性最强的乙酸乙酯提取物（EA）对突变杆状病毒有很强的抑制作用（0.390 μg/μl）。TLC 生物指纹图谱显示 F1 和 F2 点显示抑制区。F1 被鉴定为山奈酚。这是关于纤毛虫花对口腔感染的首次报道。F1 的作用模式可归因于其破坏膜完整性、减少和破坏生物膜的能力。它还能在细菌细胞内产生 ROS，导致脂质过氧化，进而导致细菌死亡：山奈酚是生物活性斑 F1 中的活性化合物，具有抗菌和抗生物膜活性。山奈酚的抗菌活性可能与山奈酚的膜破坏特性以及在变异单胞菌体内产生 ROS 有关。因此，从纤毛虫中提取的植物化学物质可用于开发药物牙科产品。

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Antibacterial and antibiofilm activities of extract and bioactive compounds from Bergenia ciliata (Haw.) Sternb. flowers against Streptococcus mutans through cell membrane damage

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Antibacterial and antibiofilm activities of extract and bioactive compounds from Bergenia ciliata (Haw.) Sternb. flowers against Streptococcus mutans through cell membrane damage

Ethnopharmacological relevance

Bergenia ciliata (Haw.) Sternb. (Family Saxifragaceae) remains mentioned as Pashanbheda in Ayurveda and Zakhmehayat in Unani. In North Waziristan, Pakistan, indigenous communities use this plant in ethnodentistry to treat tooth decay and toothaches. However, scientific evidence on its mode of action is still lacking.

Aim of the study

To evaluate the effect of extracts and fractions of B. ciliata flower against oral bacteria and elucidate the possible antibacterial and antibiofilm mechanism.

Materials and methods

Prepared extract of B. ciliata flowers were checked for its antibacterial activity against oral (S. mutans, S. pyogenes, S. oralis) and opportunistic bacteria (Staphylococcus aureus, Citrobacter clonae and Achromobacter insolitus). Preparative TLC-bioautography and silica gel column chromatography was used to isolate bioactive compounds. HRESI-MS and NMR studies were employed for its structural elucidation. Antibacterial and antibiofilm activities of extracts and isolated compounds were studied against S. mutans. Scanning Electron Microscope studies indicated membrane damage. Reactive Oxygen Species (ROS) production, lipid peroxidation and cytoplasmic leakage were also assessed.

Results

The most active ethyl acetate extract (EA) showed potent inhibitory effect against S. mutans (0.390 μg/μl). TLC–bioautography indicated spots F1 & F2 to show inhibition zones. F1 was identified as kaempferol. This is the first report on flowers of B. ciliata against oral infection. The mode of action of F1 can be attributed to its ability to destroy the membrane integrity, reducing and disrupting biofilm. It also produced ROS within the bacterial cell, leading to lipid peroxidation and subsequently causing death of the bacteria.

Conclusion

Kaempferol is the active compound in bioactive spot F1 which showed antibacterial and antibiofilm activity. The antibacterial activity can be linked with the membrane disrupting properties of kaempferol and producing ROS inside S. mutans. Thus, phytochemicals derived from B. ciliata can be used in the development of pharmaceutical dental products.

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.