联合单核染色质可及性和转录组图谱的集合 CRISPR 筛选

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2024-11-21 DOI:10.1038/s41587-024-02475-x

Rachel E. Yan, Alba Corman, Lyla Katgara, Xiao Wang, Xinhe Xue, Zoran Z. Gajic, Richard Sam, Michael Farid, Samuel M. Friedman, Jungwook Choo, Ivan Raimondi, Shridar Ganesan, Eugene Katsevich, Jeffrey P. Greenfield, Nadia Dahmane, Neville E. Sanjana

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引用次数: 0

摘要

汇集的单细胞 CRISPR 筛选可以分析基因表达或染色质可及性，但不能同时分析两种模式。在这里，我们开发了MultiPerturb-seq，这是一种高通量CRISPR筛选平台，利用组合索引技术和液滴微流控技术联合捕获单核染色质可及性、转录组和引导RNA，以提高通量并整合所有三种模式。我们确定了一种罕见儿科癌症的关键分化基因，并将 ZNHIT1 确立为癌症重编程疗法的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling

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Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling

Pooled single-cell CRISPR screens have profiled either gene expression or chromatin accessibility but not both modalities. Here we develop MultiPerturb-seq, a high-throughput CRISPR screening platform with joint single-nucleus chromatin accessibility, transcriptome and guide RNA capture using combinatorial indexing combined with droplet microfluidics to scale throughput and integrate all three modalities. We identify key differentiation genes in a rare pediatric cancer and establish ZNHIT1 as a potential target for cancer reprogramming therapy.

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

期刊介绍： Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.