长读 RNA 测序:探索人类疾病转录组复杂性的变革性技术。

IF 12.1 1区 医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Isabelle Heifetz Ament, Nicole DeBruyne, Feng Wang, Lan Lin
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引用次数: 0

摘要

长线程 RNA 测序(RNA-seq)正在成为研究人类转录组的一种强大而多用途的技术。通过对全长转录本进行端到端测序,长读RNA-seq为研究标准短读RNA-seq方法无法可靠检测的各种RNA种类和特征开辟了途径。在这篇综述中,我们概述了长读程 RNA-seq,指出了它相对于短读程 RNA-seq 的优势,并总结了实验和计算方法的最新进展,以提高基于长读程的转录组学的能力。我们描述了长读程 RNA-seq 的广泛应用,并强调了它作为探索人类疾病转录组变异的基础技术所发挥的不断扩大的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Long-read RNA sequencing: A transformative technology for exploring transcriptome complexity in human diseases.

Long-read RNA sequencing (RNA-seq) is emerging as a powerful and versatile technology for studying human transcriptomes. By enabling the end-to-end sequencing of full-length transcripts, long-read RNA-seq opens up avenues for investigating various RNA species and features that cannot be reliably interrogated by standard short-read RNA-seq methods. In this review, we present an overview of long-read RNA-seq, delineating its strengths over short-read RNA-seq, as well as summarizing recent advances in experimental and computational approaches to boost the power of long-read-based transcriptomics. We describe a wide range of applications of long-read RNA-seq, and highlight its expanding role as a foundational technology for exploring transcriptome variations in human diseases.

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来源期刊
Molecular Therapy
Molecular Therapy 医学-生物工程与应用微生物
CiteScore
19.20
自引率
3.20%
发文量
357
审稿时长
3 months
期刊介绍: Molecular Therapy is the leading journal for research in gene transfer, vector development, stem cell manipulation, and therapeutic interventions. It covers a broad spectrum of topics including genetic and acquired disease correction, vaccine development, pre-clinical validation, safety/efficacy studies, and clinical trials. With a focus on advancing genetics, medicine, and biotechnology, Molecular Therapy publishes peer-reviewed research, reviews, and commentaries to showcase the latest advancements in the field. With an impressive impact factor of 12.4 in 2022, it continues to attract top-tier contributions.
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