Yingying Lu , Huimin Liang , Jialin Liao , Xianglong Li , Yinxia Rong , Ruyin Mo , Jianmin Tang , Maoteng Li , Xiangdong Kong , Yang Huang , Yu Liang
{"title":"染色体组尺度的黄茶花(Camellia limonia)基因组组装和分析揭示喀斯特地区植物适应机制和黄酮类化合物的生物合成","authors":"Yingying Lu , Huimin Liang , Jialin Liao , Xianglong Li , Yinxia Rong , Ruyin Mo , Jianmin Tang , Maoteng Li , Xiangdong Kong , Yang Huang , Yu Liang","doi":"10.1016/j.gecco.2024.e03296","DOIUrl":null,"url":null,"abstract":"<div><div>Yellow Camellia is an endangered and protected wild plant with unique medicinal value. Among the <em>Camellia</em> Sect. <em>Chrysantha Chang</em>, certain species (e.g. <em>Camellia limonia</em>) can grow in karst regions. The karst region is a highly sensitive ecosystem with low environmental capacity. However, the molecular mechanisms underlying the adaptation of yellow camellia to karst regions remain unclear. Here, we present a high-quality genome with 15 chromosome groups with an N50 of 198.92 Mb. The divergence between <em>C. limonia</em> and <em>Camellia sinensis</em> occurred approximately 6 million years ago, indicating that the Himalayan uplift event may led to species differentiation. This enables <em>C. limonia</em> to thrive in a unique ecological environment like the karst region. Flavonoid compounds play a significant role in the interaction between plants and their environment. In comparison to Theaceae family genomes, <em>C. limonia</em> exhibits an increased number of gene family members involved in the flavonoid biosynthesis pathway, including UDP-glycosyltransferases and chalcone reductase. Additionally, two gene clusters associated with flavonoid biosynthesis were identified in the genome of <em>C. limonia</em>. Furthermore, comparative genomics analysis revealed the expansion of genes associated with karst environment adaptation in the genome of <em>C. limonia</em>, such as calmodulin genes and genes related to Calcium ion transmembrane transport. Additionally, at the gene expression level, it was observed that the secondary metabolism-related genes may be involved in the calcium tolerance of <em>C. limonia.</em> These findings provide important insights into the evolution of <em>C. limonia</em>, offering references for the study of plants in karst areas.</div></div>","PeriodicalId":54264,"journal":{"name":"Global Ecology and Conservation","volume":"56 ","pages":"Article e03296"},"PeriodicalIF":3.5000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chromosome-scale assembly and analysis of yellow Camellia (Camellia limonia) genome reveal plant adaptation mechanism and flavonoid biosynthesis in karst region\",\"authors\":\"Yingying Lu , Huimin Liang , Jialin Liao , Xianglong Li , Yinxia Rong , Ruyin Mo , Jianmin Tang , Maoteng Li , Xiangdong Kong , Yang Huang , Yu Liang\",\"doi\":\"10.1016/j.gecco.2024.e03296\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Yellow Camellia is an endangered and protected wild plant with unique medicinal value. Among the <em>Camellia</em> Sect. <em>Chrysantha Chang</em>, certain species (e.g. <em>Camellia limonia</em>) can grow in karst regions. The karst region is a highly sensitive ecosystem with low environmental capacity. However, the molecular mechanisms underlying the adaptation of yellow camellia to karst regions remain unclear. Here, we present a high-quality genome with 15 chromosome groups with an N50 of 198.92 Mb. The divergence between <em>C. limonia</em> and <em>Camellia sinensis</em> occurred approximately 6 million years ago, indicating that the Himalayan uplift event may led to species differentiation. This enables <em>C. limonia</em> to thrive in a unique ecological environment like the karst region. Flavonoid compounds play a significant role in the interaction between plants and their environment. In comparison to Theaceae family genomes, <em>C. limonia</em> exhibits an increased number of gene family members involved in the flavonoid biosynthesis pathway, including UDP-glycosyltransferases and chalcone reductase. Additionally, two gene clusters associated with flavonoid biosynthesis were identified in the genome of <em>C. limonia</em>. Furthermore, comparative genomics analysis revealed the expansion of genes associated with karst environment adaptation in the genome of <em>C. limonia</em>, such as calmodulin genes and genes related to Calcium ion transmembrane transport. Additionally, at the gene expression level, it was observed that the secondary metabolism-related genes may be involved in the calcium tolerance of <em>C. limonia.</em> These findings provide important insights into the evolution of <em>C. limonia</em>, offering references for the study of plants in karst areas.</div></div>\",\"PeriodicalId\":54264,\"journal\":{\"name\":\"Global Ecology and Conservation\",\"volume\":\"56 \",\"pages\":\"Article e03296\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-11-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Global Ecology and Conservation\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2351989424005006\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIODIVERSITY CONSERVATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Global Ecology and Conservation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2351989424005006","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
引用次数: 0
摘要
黄茶花是一种濒临灭绝的受保护野生植物,具有独特的药用价值。在山茶花科(Camellia Sect.中,某些品种(如山茶花)可以在岩溶地区生长。岩溶地区是一个高度敏感的生态系统,环境容量较低。然而,黄茶花适应喀斯特地区的分子机制仍不清楚。在此,我们展示了一个包含 15 个染色体组、N50 为 198.92 Mb 的高质量基因组。黄山茶和山茶花的分化发生在大约600万年前,这表明喜马拉雅山隆升事件可能导致了物种分化。这使得 C. limonia 能够在喀斯特地区这样一个独特的生态环境中茁壮成长。类黄酮化合物在植物与其环境的相互作用中发挥着重要作用。与菊科植物基因组相比,C. limonia表现出更多参与类黄酮生物合成途径的基因家族成员,包括UDP-糖基转移酶和查尔酮还原酶。此外,在 C. limonia 的基因组中还发现了两个与类黄酮生物合成相关的基因簇。此外,比较基因组学分析表明,C. limonia 基因组中与岩溶环境适应有关的基因有所增加,如钙调蛋白基因和与钙离子跨膜转运有关的基因。此外,在基因表达水平上观察到,次生代谢相关基因可能参与了 C. limonia 的钙耐受性。这些发现为研究石灰华的进化提供了重要的启示,为岩溶地区植物的研究提供了参考。
Chromosome-scale assembly and analysis of yellow Camellia (Camellia limonia) genome reveal plant adaptation mechanism and flavonoid biosynthesis in karst region
Yellow Camellia is an endangered and protected wild plant with unique medicinal value. Among the Camellia Sect. Chrysantha Chang, certain species (e.g. Camellia limonia) can grow in karst regions. The karst region is a highly sensitive ecosystem with low environmental capacity. However, the molecular mechanisms underlying the adaptation of yellow camellia to karst regions remain unclear. Here, we present a high-quality genome with 15 chromosome groups with an N50 of 198.92 Mb. The divergence between C. limonia and Camellia sinensis occurred approximately 6 million years ago, indicating that the Himalayan uplift event may led to species differentiation. This enables C. limonia to thrive in a unique ecological environment like the karst region. Flavonoid compounds play a significant role in the interaction between plants and their environment. In comparison to Theaceae family genomes, C. limonia exhibits an increased number of gene family members involved in the flavonoid biosynthesis pathway, including UDP-glycosyltransferases and chalcone reductase. Additionally, two gene clusters associated with flavonoid biosynthesis were identified in the genome of C. limonia. Furthermore, comparative genomics analysis revealed the expansion of genes associated with karst environment adaptation in the genome of C. limonia, such as calmodulin genes and genes related to Calcium ion transmembrane transport. Additionally, at the gene expression level, it was observed that the secondary metabolism-related genes may be involved in the calcium tolerance of C. limonia. These findings provide important insights into the evolution of C. limonia, offering references for the study of plants in karst areas.
期刊介绍:
Global Ecology and Conservation is a peer-reviewed, open-access journal covering all sub-disciplines of ecological and conservation science: from theory to practice, from molecules to ecosystems, from regional to global. The fields covered include: organismal, population, community, and ecosystem ecology; physiological, evolutionary, and behavioral ecology; and conservation science.