分散红 1 偶氮染料：小鼠和斑马鱼低剂量/低浓度接触的后果

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-11-01 DOI:10.1016/j.mrgentox.2024.503830

Fábio Henrique Fernandes , Flávia Renata Abe , Tamara Barbosa Gomes , Cibele Borges dos Santos , Wilma De Grava Kempinas , Bianca Arruda Leite , Danielle Palma de Oliveira , Daisy Maria Fávero Salvadori

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引用次数: 0

摘要

颜色指数分散红 1（DR1）是一种广泛用于纺织业并被排放到水生环境中的偶氮染料，在体细胞中具有遗传毒性，但人们对其对生殖系统或胚胎发育早期阶段的影响知之甚少。我们评估了雄性小鼠口服低剂量染料 14 天后精子受到的影响。测量终点包括 DNA 损伤（彗星试验）、miRNA-34c 水平以及精子数量、形态和活力。暴露会导致 miRNA-34c 水平下降。我们还研究了染料对斑马鱼胚胎和幼虫的影响，包括发育障碍、谷胱甘肽转移酶活性改变以及对活性氧和脂质过氧化水平的影响。

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Disperse Red 1 azo dye: Consequences of low-dose/low-concentration exposures in mice and zebrafish

Color Index Disperse Red 1 (DR1), an azo dye widely used in the textile industry and released into aquatic environments, is genotoxic in somatic cells, but little is known concerning its effects on the reproductive system or the early stages of embryonic development. We have assessed the effects on the spermatozoa of male mice following oral exposure to the dye, at low doses, for 14 days. Measured endpoints were DNA damage (comet assay), miRNA-34c levels, and sperm number, morphology, and motility. Exposure caused decreased miRNA-34c levels. We have also examined dye effects on zebrafish embryos and larvae, which included developmental impairment, altered glutathione transferase activity, and effects on reactive oxygen species and lipid peroxidation levels.

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.