用于无标记、可靠检测妊娠糖尿病相关 miRNA (miR-135a)的双模式、信号放大 DNA 生物传感器。

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2024-11-12 DOI:10.1016/j.jpba.2024.116565

Jiang-Nan Zhang, Feng-Min Liu, Xiao-Juan Du, Xi-Le Zhao

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引用次数: 0

摘要

miR-135a 在妊娠糖尿病患者中高表达，其靶基因也参与了胰岛素信号通路，因此是妊娠糖尿病的生物标志物之一。在此，我们设计了一种基于荧光和比色信号的双模式 DNA 生物传感器，用于可靠地检测 miR-135a。为了证明这种双模式 DNA 生物传感器的检测可行性和机制，我们进行了多项实验。通过优化参数，该双模式生物传感器实现了对 miR-135a 的灵敏定量检测。荧光信号和比色信号的工作范围分别为 0.56-61 nM 和 8.3-74 nM，检测限分别为 0.18 nM 和 3.7 nM。这种双模式策略使 miR-135a 检测具有两个独立的信号，因此可以相互验证，从而显示出更准确、更可靠的结果。此外，该生物传感器对目标 miR-135a 而不是其他核苷酸变体具有良好的选择性，在复杂样品中也具有良好的抗干扰能力。这种双模式 DNA 生物传感器为妊娠糖尿病的 miR-135a 检测和 miRNA 表达谱分析提供了一种新方法。

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Dual-mode, signal-amplified DNA biosensor for label-free, reliable assay of gestational diabetes mellitus-related miRNA (miR-135a)

miR-135a is highly expressed in patients with gestational diabetes mellitus, and its target genes are also involved in insulin signaling pathway, so it is one biomarker for gestational diabetes mellitus. Herein we designed a dual-mode DNA biosensor for reliable assay of miR-135a based on the fluorescence and colorimetric signals. Several experiments were carried out to demonstrate the assay feasibility and mechanism for this dual-mode DNA biosensor. With optimum parameters, this proposed dual-mode biosensor has been realized sensitive and quantitative assay of miR-135a. For the fluorescence and colorimetric signals, the working ranges are 0.56–61 and 8.3–74 nM, while limits of detection are 0.18 and 3.7 nM respectively. This dual-mode strategy allows two independent signals for miR-135a assay, so it can verify each other to show more accurate results with good fidelity. Furthermore, there is a good selectivity in the biosensor for target miR-135a over other nucleotide variants, as well as good anti-interference ability in complex samples. This dual-mode DNA biosensor provides a new approach for miR-135a assay and miRNA expression profiling in gestational diabetes mellitus.

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.