短链脂肪酸对 TNF-α 诱导的肠道氧化应激的保护作用

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Miguel Ferrer , Berta Buey , Laura Grasa , Jose Emilio Mesonero , Eva Latorre
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引用次数: 0

摘要

炎症性肠病(IBD)是由夸张的炎症反应引起的,它会导致氧化应激明显增加。这反过来又加剧了炎症过程,造成严重的细胞和组织损伤。肠道菌群失调是 IBD 患者的常见症状,它会改变细菌代谢产物的产生,包括膳食纤维发酵的主要副产品短链脂肪酸 (SCFA)。虽然 SCFAs 在肠道生理学中的作用仍有待阐明,但本研究旨在调查它们对肠道氧化应激的影响,尤其是在促炎介质肿瘤坏死因子α(TNF-α)诱导的炎症条件下。我们采用 Caco-2/TC7 细胞系作为肠上皮细胞的体外模型,并用一系列 SCFAs(包括乙酸盐、丙酸盐和丁酸盐)对细胞进行处理。对蛋白质和脂质氧化水平以及抗氧化酶的活性进行了量化。我们的研究结果表明,微生物群衍生的 SCFAs 可通过调节抗氧化酶的活性,有效缓解 TNF-α 诱导的氧化应激。促炎介质 TNF-α 通过抑制过氧化氢酶和谷胱甘肽过氧化物酶的活性来诱导脂质过氧化。SCFAs 能够上调抗氧化酶的活性,从而恢复脂质氧化水平。这些结果强调了肠道微生物群在维持肠道平衡中的关键作用,并突出了 SCFAs 在控制氧化应激相关病症方面的治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective role of short-chain fatty acids on intestinal oxidative stress induced by TNF-α
Inflammatory bowel diseases (IBDs) are driven by an exaggerated inflammatory response, which leads to a marked increase in oxidative stress. This, in turn, exacerbates the inflammatory process and causes significant cellular and tissue damage. Intestinal dysbiosis, a common observation in IBD patients, alters the production of bacterial metabolites, including short-chain fatty acids (SCFAs), which are key by-products of dietary fiber fermentation. While the role of SCFAs in intestinal physiology is still being elucidated, this study aimed to investigate their effects on intestinal oxidative stress, particularly under inflammatory conditions induced by the proinflammatory mediator tumor necrosis factor alpha (TNF-α). The Caco-2/TC7 cell line was employed as an in vitro model of the intestinal epithelium, and the cells were treated with a range of SCFAs, including acetate, propionate, and butyrate. The levels of protein and lipid oxidation were quantified, as well as the activity of antioxidant enzymes. Our findings demonstrate that microbiota-derived SCFAs can effectively mitigate TNF-α-induced oxidative stress by modulating antioxidant enzyme activity. The proinflammatory mediator TNF-α induces lipid peroxidation by inhibiting catalase and glutathione peroxidase activities. SCFAs are able to upregulate antioxidant enzyme activity to restore lipid oxidative levels. These results underscore the critical role of the gut microbiota in maintaining intestinal homeostasis and highlight the therapeutic potential of SCFAs in managing oxidative stress-related pathologies.
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来源期刊
Cell Stress & Chaperones
Cell Stress & Chaperones 生物-细胞生物学
CiteScore
7.60
自引率
2.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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