基于平板测量分离线粒体和完整细胞的呼吸作用

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4264-1_3

Shona A Mookerjee

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引用次数: 0

摘要

测量呼吸速率是评估离体线粒体和完整细胞能量功能的有力方法。与老式的悬浮系统相比，目前基于平板的方法有几个优点，包括更高的通量和只需微克量的材料。在本章中，我们将对之前发表的在 96 孔板中以平板为基础测量分离的粘附线粒体耗氧量的方法进行更新。我们还介绍了在 96 孔板中对完整细胞进行相同测量的方法。

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Plate-Based Measurement of Respiration by Isolated Mitochondria and by Intact Cells.

Measuring respiration rate can be a powerful way to assess energetic function in isolated mitochondria and intact cells. Current plate-based methods have several advantages over older suspension-based systems, including greater throughput and the requirement of only microgram quantities of material. In this chapter, we provide an update to our previously published methods for plate-based measurement of oxygen consumption in isolated adherent mitochondria in a 96-well format plate. We also describe methods for performing the same measurements on intact cells in a 96-well format.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.