联合 PCPE-1 和α-胎儿蛋白对肝细胞癌的预后意义。

IF 5 2区医学 Q2 Medicine

Translational Oncology Pub Date : 2024-11-09 DOI:10.1016/j.tranon.2024.102185

Ruhua Zhang , Wanqi Chen , Xuelan Peng , Zhiguang Zhang , Shangjiu Yang , Li Zhong

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引用次数: 0

摘要

目的：前胶原蛋白C蛋白酶增强子1（PCPE-1）与肝纤维化有关，而肝纤维化是肝细胞癌（HCC）的主要风险因素。然而，它在 HCC 中的作用仍不清楚：使用公开的 HCC 组织和匹配的正常组织数据集分析 PCPE-1 的 mRNA 和蛋白表达水平。用 Western 印迹法测定了 7 个配对的 HCC 肿瘤和正常组织中的 PCPE-1 水平。免疫组化法检测了 155 例 HCC 患者的 PCPE-1 水平。采用 ROC 曲线确定 PCPE-1 的最佳临界值。单变量和多变量分析确定了与HCC患者总生存期（OS）相关的独立风险因素。Kaplan-Meier分析评估了PCPE-1表达与OS之间的关系，并构建了一个预后模型：结果：与正常组织相比，PCPE-1蛋白在HCC组织中上调，并与几种原胶原的表达呈正相关。在155例HCC患者中，78例患者的PCPE-1胞浆染色表达升高。PCPE-1 的高表达与肿瘤坏死（P = 0.045）、较差的组织学分级（G3-G4，P = 0.008）和较高的α-胎儿蛋白（AFP）水平（>20 ng/ml，P = 0.043）明显相关。单变量和多变量分析均显示，PCPE-1升高与较差的总生存率之间存在显著关联（两项分析中的P均小于0.001）。值得注意的是，PCPE-1和AFP联合预后模型能有效地对HCC的OS风险进行分层：我们的研究结果首次证明，PCPE-1是HCC的独立预后标志物，PCPE-1和AFP联合预后模型是预测患者预后的重要工具。

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Prognostic significance of combined PCPE-1 and α-fetoprotein for hepatocellular carcinoma

Purpose

Procollagen C-proteinase enhancer 1 (PCPE-1) is associated with liver fibrosis, a major risk factor of hepatocellular carcinoma (HCC). However, its role in HCC remains unclear.

Materials and Methods

The mRNA and protein expression levels of PCPE-1 were analyzed using publicly available datasets of HCC tissues and matched normal tissues. Western blotting was performed to determine PCPE-1 levels in 7 paired HCC tumor and normal tissues. Immunohistochemistry was used to detect PCPE-1 levels in 155 HCC patients. The ROC curve was employed to determine the optimal cutoff value of PCPE-1. Univariate and multivariate analyses identified independent risk factors associated with overall survival (OS) of HCC patients. Kaplan-Meier analysis assessed the relationship between PCPE-1 expression and OS, and a prognostic model was constructed.

Results

PCPE-1 protein was upregulated in HCC tissues compared to normal tissues and positively correlated with the expression of several procollagens. 78 out of 155 HCC patients exhibited elevated PCPE-1 expression with cytoplasmic staining. High PCPE-1 expression significantly correlated with tumor necrosis (P = 0.045), poorer histologic grade (G3-G4, P = 0.008), and higher α-fetoprotein (AFP) level (>20 ng/ml, P = 0.043). Both univariate and multivariate analyses showed a significant association between elevated PCPE-1 and poorer overall survival (P < 0.001 in both analyses). Remarkably, combined prognostic model with PCPE-1 and AFP effectively stratified the risk for OS in HCC.

Conclusion

Our results demonstrate for the first time that PCPE-1 serves as an independent prognostic marker for HCC, and the combined prognostic model involving PCPE-1 and AFP emerges as a valuable tool for predicting patient outcomes.

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来源期刊

Translational Oncology ONCOLOGY-

CiteScore

8.40

自引率

2.00%

发文量

314

审稿时长

54 days

期刊介绍： Translational Oncology publishes the results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of oncology patients. Translational Oncology will publish laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer. Peer reviewed manuscript types include Original Reports, Reviews and Editorials.