具有慢性伤口特征的体外延迟划痕闭合试验

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-11-10 DOI:10.1007/7651_2024_578

Aimin Yeo, Yi Bing Aw, Pamela Mok

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引用次数: 0

摘要

划痕试验是一种用于评估细胞迁移的简单伤口愈合模型，在该模型中，汇合的细胞层被划伤，形成划痕区（"伤口"），细胞迁移到划痕区。在这里，我们描述了一种划痕试验，它表现出延迟闭合和炎症加重，与慢性伤口更为相似。与更接近急性伤口的传统划痕伤口试验相比，这种延迟划痕闭合试验更适用于筛选化合物和研究延迟伤口愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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An In Vitro Delayed Scratch Closure Assay with Features of Chronic Wounds.

A scratch assay is a simple wound healing model for assessing cell migration in which a confluent cell layer is scratched to create a scratch zone ("wound") that cells migrate into. Here, we describe a scratch assay exhibiting delayed closure and increased inflammation, which more closely resemble chronic wounds. This delayed scratch closure assay is more relevant for screening compounds and studying delayed wound healing compared with traditional scratch wound assays that more closely resemble acute wounds.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.