揭开荠菜 MBOAT 基因家族的神秘面纱:系统发育见解和调控格局。

IF 2.6 3区 生物学 Q2 GENETICS & HEREDITY
Gene Pub Date : 2024-11-13 DOI:10.1016/j.gene.2024.149085
Pengfang Qiao, Maoqiu Zhao, Jialiang Zhao, Jiayin Wen, Cuizhu Zhao, Meng Zhang
{"title":"揭开荠菜 MBOAT 基因家族的神秘面纱:系统发育见解和调控格局。","authors":"Pengfang Qiao,&nbsp;Maoqiu Zhao,&nbsp;Jialiang Zhao,&nbsp;Jiayin Wen,&nbsp;Cuizhu Zhao,&nbsp;Meng Zhang","doi":"10.1016/j.gene.2024.149085","DOIUrl":null,"url":null,"abstract":"<div><div>The membrane-bound O-acyltransferase (MBOAT) gene family comprises enzymes responsible for transferring acyl groups to various lipid molecules. Some members of the MBOAT gene family and their functions have been extensively studied in the model plant Arabidopsis. However, research on the MBOAT gene family in camelina is still limited. In this study, 54 MBOATs were identified on 17 chromosomes and one unidentified scaffold in camelina, including seven newly identified genes. A total of 149 MBOATs were identified in 10 other species. Six subgroups of these MBOATs with different conservation were classified by phylogenetic analysis, showing diversification between monocots and dicots. Detailed analysis of the motif composition, evolutionary relationships, and gene structures of CsaMBOATs are presented. The results of the syntenic analysis suggest that the evolution of CsaMBOAT gene family is primarily driven by segmental and tandem duplications, and that there is a stronger collinearity within dicots. In addition, analysis of CsaMBOAT gene promoter <em>cis</em>-elements reveals a possible transcriptional regulation and tissue-specific expression, highlighting potential role in plant stress responses and hormone signaling. Furthermore, both the transcriptome and RT-qPCR data revealed the changes in the expression levels of <em>DGAT1</em> during salt stress treatment. Subsequent analyses indicated that <em>DGAT1</em> influenced the ratio of fatty acid fractions in the plants. Importantly, a large number of transcription factors involved in the regulation of CsaMBOAT gene expression were identified by WGCNA analysis, and the transcriptional data confirmed that the <em>NAC032</em> and <em>CAMMTA6</em> genes play a role upstream of <em>DGAT1</em>. This study not only identified the members of the MBOAT in camelina, but also provided insights and clues into its regulatory mechanisms.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"936 ","pages":"Article 149085"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unveiling the camelina MBOAT gene family: Phylogenetic insights and regulatory landscape\",\"authors\":\"Pengfang Qiao,&nbsp;Maoqiu Zhao,&nbsp;Jialiang Zhao,&nbsp;Jiayin Wen,&nbsp;Cuizhu Zhao,&nbsp;Meng Zhang\",\"doi\":\"10.1016/j.gene.2024.149085\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The membrane-bound O-acyltransferase (MBOAT) gene family comprises enzymes responsible for transferring acyl groups to various lipid molecules. Some members of the MBOAT gene family and their functions have been extensively studied in the model plant Arabidopsis. However, research on the MBOAT gene family in camelina is still limited. In this study, 54 MBOATs were identified on 17 chromosomes and one unidentified scaffold in camelina, including seven newly identified genes. A total of 149 MBOATs were identified in 10 other species. Six subgroups of these MBOATs with different conservation were classified by phylogenetic analysis, showing diversification between monocots and dicots. Detailed analysis of the motif composition, evolutionary relationships, and gene structures of CsaMBOATs are presented. The results of the syntenic analysis suggest that the evolution of CsaMBOAT gene family is primarily driven by segmental and tandem duplications, and that there is a stronger collinearity within dicots. In addition, analysis of CsaMBOAT gene promoter <em>cis</em>-elements reveals a possible transcriptional regulation and tissue-specific expression, highlighting potential role in plant stress responses and hormone signaling. Furthermore, both the transcriptome and RT-qPCR data revealed the changes in the expression levels of <em>DGAT1</em> during salt stress treatment. Subsequent analyses indicated that <em>DGAT1</em> influenced the ratio of fatty acid fractions in the plants. Importantly, a large number of transcription factors involved in the regulation of CsaMBOAT gene expression were identified by WGCNA analysis, and the transcriptional data confirmed that the <em>NAC032</em> and <em>CAMMTA6</em> genes play a role upstream of <em>DGAT1</em>. This study not only identified the members of the MBOAT in camelina, but also provided insights and clues into its regulatory mechanisms.</div></div>\",\"PeriodicalId\":12499,\"journal\":{\"name\":\"Gene\",\"volume\":\"936 \",\"pages\":\"Article 149085\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0378111924009661\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378111924009661","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

摘要

膜结合 O-酰基转移酶(MBOAT)基因家族由负责将酰基转移到各种脂质分子的酶组成。人们在模式植物拟南芥中对 MBOAT 基因家族的一些成员及其功能进行了广泛研究。然而,对荠菜中 MBOAT 基因家族的研究仍然有限。本研究在荠菜的 17 条染色体和 1 个未确定的支架上鉴定了 54 个 MBOAT,其中包括 7 个新鉴定的基因。在其他 10 个物种中共鉴定出 149 个 MBOATs。通过系统发育分析,这些 MBOATs 的六个亚群具有不同的保存方式,显示出单子叶植物和双子叶植物之间的多样化。本文详细分析了 CsaMBOATs 的主题词组成、进化关系和基因结构。同源分析结果表明,CsaMBOAT 基因家族的进化主要是由节段复制和串联复制驱动的,而且在双子叶植物中存在更强的共线性。此外,对 CsaMBOAT 基因启动子顺式元件的分析揭示了可能的转录调控和组织特异性表达,突出了其在植物胁迫响应和激素信号转导中的潜在作用。此外,转录组和 RT-qPCR 数据都揭示了 DGAT1 在盐胁迫处理过程中的表达水平变化。随后的分析表明,DGAT1 影响了植物中脂肪酸组分的比例。重要的是,通过 WGCNA 分析发现了大量参与调控 CsaMBOAT 基因表达的转录因子,转录数据证实 NAC032 和 CAMMTA6 基因在 DGAT1 的上游发挥作用。这项研究不仅确定了荠菜中 MBOAT 的成员,还为其调控机制提供了见解和线索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling the camelina MBOAT gene family: Phylogenetic insights and regulatory landscape
The membrane-bound O-acyltransferase (MBOAT) gene family comprises enzymes responsible for transferring acyl groups to various lipid molecules. Some members of the MBOAT gene family and their functions have been extensively studied in the model plant Arabidopsis. However, research on the MBOAT gene family in camelina is still limited. In this study, 54 MBOATs were identified on 17 chromosomes and one unidentified scaffold in camelina, including seven newly identified genes. A total of 149 MBOATs were identified in 10 other species. Six subgroups of these MBOATs with different conservation were classified by phylogenetic analysis, showing diversification between monocots and dicots. Detailed analysis of the motif composition, evolutionary relationships, and gene structures of CsaMBOATs are presented. The results of the syntenic analysis suggest that the evolution of CsaMBOAT gene family is primarily driven by segmental and tandem duplications, and that there is a stronger collinearity within dicots. In addition, analysis of CsaMBOAT gene promoter cis-elements reveals a possible transcriptional regulation and tissue-specific expression, highlighting potential role in plant stress responses and hormone signaling. Furthermore, both the transcriptome and RT-qPCR data revealed the changes in the expression levels of DGAT1 during salt stress treatment. Subsequent analyses indicated that DGAT1 influenced the ratio of fatty acid fractions in the plants. Importantly, a large number of transcription factors involved in the regulation of CsaMBOAT gene expression were identified by WGCNA analysis, and the transcriptional data confirmed that the NAC032 and CAMMTA6 genes play a role upstream of DGAT1. This study not only identified the members of the MBOAT in camelina, but also provided insights and clues into its regulatory mechanisms.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Gene
Gene 生物-遗传学
CiteScore
6.10
自引率
2.90%
发文量
718
审稿时长
42 days
期刊介绍: Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信