巨型积雪草是一种富含积雪草苷和积雪草苷的新型栽培品种,它能通过与 MC1R 结合抑制α-黑色素细胞刺激素诱导的黑色素生成。

IF 5.7 3区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
International journal of molecular medicine Pub Date : 2025-01-01 Epub Date: 2024-11-08 DOI:10.3892/ijmm.2024.5454
Jiwon Seo, Chanhyeok Jeong, Seung Man Oh, Sung-Young Lee, Han Woong Park, Dae Bang Seo, Dae Sung Yoo, Woo-Jin Sim, Tae-Gyu Lim, Jung Han Yoon Park, Chang Hyung Lee, Ki Won Lee
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引用次数: 0

摘要

本研究调查了巨型积雪草(Giant Centella asiatica,GCA)的抗黑色素生成效果,并将其与传统积雪草的功效进行了比较。GCA 的单位面积产量高,抗氧化性更强。研究人员使用 B16F10 黑色素瘤细胞和三维人体皮肤等效模型研究了 GCA 的抗黑色素生成作用。通过 Western 印迹、cAMP 检测和分子对接研究阐明了关键的分子机制。通过比较 GCA 提取物与 CA 提取物抑制 B16F10 黑色素瘤细胞和三维人体皮肤等效模型中黑色素生成的能力,重点研究了 GCA 对皮肤美白的影响。结果表明,GCA 提取物能更有效地减少黑色素的生成,这要归功于其较高含量的两种活性成分--马黛茶苷和积雪草苷。进一步的研究发现,GCA主要通过PKA-CAMP反应元件结合(CREB)-微眼炎相关转录因子(MITF)轴抑制黑色素生成,而该轴是黑色素合成的关键调控途径。值得注意的是,据我们所知,本研究首次证明了 GCA 中的两种主要化合物--马黛茶苷和积雪草苷能直接与 MC1R 结合,从而产生显著的美白效果。此外,GCA 在三维人体皮肤等效模型中减少了黑色素的生成,显示了在复杂皮肤环境中的功效。这些结果表明,在抗皮肤黑色素生成方面,GCA 的效果优于 CA,这表明它有望成为一种针对色素沉着疾病的天然材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Giant Centella asiatica, a novel cultivar rich in madecassoside and asiaticoside, suppresses α‑melanocyte‑stimulating hormone‑induced melanogenesis through MC1R binding.

The present study investigated the anti‑melanogenesis effects of Giant Centella asiatica (GCA), a new cultivator of Centella asiatica (CA) cataloged by the Korea Forest Service in 2022, and compared its efficacy with that of traditional CA. GCA has a high yield per unit area and enhanced antioxidant properties. The anti‑melanogenic effects of GCA were investigated using B16F10 melanoma cells and a 3D human skin‑equivalent model. Key molecular mechanisms were elucidated through western blotting, cAMP assays and molecular docking studies. Focus was addressed on the effect of GCA on skin whitening by comparing the ability of a GCA extract to inhibit melanin production in B16F10 melanoma cells and a 3D human skin‑equivalent model to that of CA. The results showed that the GCA extracts more effectively reduced melanin production, which was attributed to their higher content of two active components, madecassoside and asiaticoside. Further investigation revealed that GCA primarily inhibited melanogenesis through the PKA‑cAMP response element‑binding (CREB)‑microphthalmia‑associated transcription factor (MITF) axis, a key regulatory pathway in melanin synthesis. Notably, the present study, to the best of our knowledge, is the first to demonstrate that madecassoside and asiaticoside, the two principal compounds in GCA, directly bound to MC1R, which contributed to the significant skin‑whitening effects. Moreover, GCA reduced melanin production in a 3D human skin‑equivalent model, showing efficacy within a complex skin environment. These results demonstrated the superior effectiveness of GCA to that of CA for skin anti‑melanogenesis, indicating its potential as a promising natural material for targeting pigmentation disorders.

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来源期刊
International journal of molecular medicine
International journal of molecular medicine 医学-医学:研究与实验
CiteScore
12.30
自引率
0.00%
发文量
124
审稿时长
3 months
期刊介绍: The main aim of Spandidos Publications is to facilitate scientific communication in a clear, concise and objective manner, while striving to provide prompt publication of original works of high quality. The journals largely concentrate on molecular and experimental medicine, oncology, clinical and experimental cancer treatment and biomedical research. All journals published by Spandidos Publications Ltd. maintain the highest standards of quality, and the members of their Editorial Boards are world-renowned scientists.
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