通过 IR-MALDESI 质谱成像技术利用 2940 nm 激光获得 20 μm 的空间分辨率。

IF 3.1 2区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of the American Society for Mass Spectrometry Pub Date : 2024-12-04 Epub Date: 2024-11-08 DOI:10.1021/jasms.4c00276

Seth M Eisenberg, Alena N Joignant, Kevan T Knizner, Jeffrey G Manni, David C Muddiman

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引用次数: 0

摘要

高空间分辨率是质谱成像（MSI）的一个关键参数，它有助于更好地了解系统生物学和细胞过程。利用具有良好高斯光束质量（M2 = 4）的新型红外激光器、空间滤波和反射物镜，IR-MALDESI 获得了 20 μm 的空间分辨率。在证明肝脏组织成像的可行性之前，在烧纸上对光学系统进行了优化。最后，利用空间分辨率分别为 20 微米和 140 微米的嵌套感兴趣区对小鼠大脑进行了分析，以较高的空间分辨率检测了胼胝体和周围脑组织的神经递质和脂质。

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Obtaining 20 μm Spatial Resolution with a 2940 nm Laser by IR-MALDESI Mass Spectrometry Imaging.

High spatial resolution is a key parameter in mass spectrometry imaging (MSI), enabling a greater understanding of system biology and cellular processes. Using a novel IR laser with good Gaussian beam quality (M² = 4) coupled with spatial filtering and a reflective objective, 20 μm spatial resolution was obtained by IR-MALDESI. The optical train was optimized on burn paper before demonstrating feasibility for imaging of liver tissue. Finally, a mouse brain was analyzed using nested regions of interest at 20 and 140 μm spatial resolution, detecting neurotransmitters and lipids with high spatial resolution on the corpus callosum and surrounding brain tissue.

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来源期刊

Journal of the American Society for Mass Spectrometry 化学-分析化学

CiteScore

5.50

自引率

9.40%

发文量

257

审稿时长

1 months

期刊介绍： The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives