François Brinas, Nicolas Sailliet, Gaëlle Tilly, Laurence Delbos, Clarisse Kerleau, Magali Giral, Nicolas Degauque, Sophie Brouard, Richard Danger
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引用次数: 0
摘要
尽管有极少数受者无需免疫抑制治疗即可获得长期稳定的移植物,他们被称为手术耐受患者(TOLs),但即使在肾移植术后很长一段时间内,也需要使用免疫抑制治疗来防止排斥反应的发生。我们全面研究了长期肾移植受者(LTTs)和手术耐受患者(TOLs)的免疫系统,以突出他们共同和独特的免疫特征。血液免疫细胞表型分析是通过光谱细胞仪进行的。分析了 34 人的样本,包括 6 名 LTT、8 名 TOL、10 名移植后 1 年的稳定期患者(STA)和 10 名健康志愿者。LTT和TOL患者的B细胞不同,LTT患者的B细胞总频率降低,并获得记忆表型,而TOL患者则保持天真表型。在 LTTs 中,IgD-CD27- B 细胞和 CD11c+ 记忆 B 细胞的频率增加,表型衰竭,CD25 表达显著下降。这些 CD11c+ B 细胞表现出的衰竭表型与几种慢性免疫性疾病中发现的表型相似,而在这些疾病中,CD11c+ B 细胞被证明参与了病理生理学,这表明 LTTs 中存在活跃的慢性炎症。总之,这些数据表明,应采取预防措施尽量减少 IS 的使用。
Rise of a CD27− IgD− CD11c+ B cells population in kidney recipients achieving long-term graft stability under immunosuppression
The use of immunosuppressive treatment is required to prevent rejection events, even a long time after kidney transplantation despite rare recipients achieving long-term graft stability without the need for immunosuppressive treatment, called operationally tolerant patients (TOLs). We comprehensively investigate the immune system of long-term IS recipients (LTTs) and TOLs to highlight their shared and unique immune features. Blood immune cell phenotyping was performed by spectral cytometry. Samples from 34 individuals were analyzed, including 6 LTTs, 8 TOLs, 10 stable patients at 1 year posttransplantation (STAs), and 10 healthy volunteers. B cells differed between LTTs and TOLs with a decreased total B-cell frequency and the acquisition of a memory phenotype in LTTs whereas a naive phenotype is maintained in TOLs. The frequencies of IgD−CD27− B cells and CD11c+ memory B cells are increased in LTTs, with an exhausted phenotype, evoked by a significant decrease in CD25 expression. These CD11c+ B cells display an exhausted phenotype similar to those found in several chronic immune diseases in which they have been shown to participate in their pathophysiology, suggesting active chronic inflammation in LTTs. Altogether, these data indicate that precautions should be taken to minimize IS use.
期刊介绍:
The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.