单次静脉注射溶瘤腺病毒 TILT-123 可使晚期实体瘤患者产生全身肿瘤转导和免疫反应。

IF 11.4 1区 医学 Q1 ONCOLOGY
Elise Jirovec, Dafne C A Quixabeira, James H A Clubb, Santeri A Pakola, Tatiana Kudling, Victor Arias, Lyna Haybout, Katriina Jalkanen, Tuomo Alanko, Tine Monberg, Amir Khammari, Brigitte Dreno, Inge Marie Svane, Matthew S Block, Daniel A Adamo, Johanna Mäenpää, Claudia Kistler, Suvi Sorsa, Otto Hemminki, Anna Kanerva, João M Santos, Victor Cervera-Carrascon, Akseli Hemminki
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引用次数: 0

摘要

背景:已获批准的溶瘤病毒的一个局限性是需要进行瘤内注射。TILT-123(igrelimogene litadenorepvec, Ad5/3-E2F-D24-hTNFα-IRES-hIL-2)是一种嵌合型溶瘤腺病毒,由于其病毒帽修饰和双选择性装置,适合静脉注射。它含有肿瘤坏死α和白细胞介素-2,可促进T细胞活化和淋巴细胞迁移到肿瘤,从而增强抗肿瘤免疫反应。在此,我们将介绍在三项 I 期剂量递增临床试验中单次静脉注射 TILT-123 后的结果:方法:晚期实体瘤患者最初接受单次静脉注射剂量为 3 × 109 至 4 × 1012 的 TILT-123 病毒颗粒(VP)。在基线、治疗后1、16和192小时(7天)采集血液,进行生物利用度和血清分析。在治疗前和治疗后 7 天收集肿瘤活检组织,以分析病毒的存在和免疫效应。在此期间,患者没有接受任何其他癌症疗法:在所有三项试验(TUNIMO、TUNINTIL 和 PROTA)中,共有 52 名患者接受了静脉注射 TILT-123 治疗。总体而言,TILT-123 的耐受性良好,未发现剂量限制性毒性反应。治疗后的肿瘤活检显示病毒基因的表达、TILT-123腺病毒蛋白或DNA的存在以及免疫细胞浸润与基线相比的变化。病毒剂量的增加并未导致肿瘤中病毒检测的增加。治疗后活检证实存在TILT-123的患者中位总生存期更长(280天对190天,P = 0.0405):结论:TILT-123在单次静脉给药后表现出安全性和显著的瘤内免疫调节作用,值得进一步研究:试验注册:TUNIMO-NCT04695327。2021 年 1 月 4 日注册,https://clinicaltrials.gov/study/NCT04695327 。TUNINTIL-NCT04217473.注册时间为 2019 年 12 月 19 日,https://clinicaltrials.gov/study/NCT04217473 。PRTA-NCT05271318。2022 年 2 月 4 日注册,https://clinicaltrials.gov/study/NCT05271318 。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single intravenous administration of oncolytic adenovirus TILT-123 results in systemic tumor transduction and immune response in patients with advanced solid tumors.

Background: A limitation of approved oncolytic viruses is their requirement for intratumoral (i.t.) injection. TILT-123 (igrelimogene litadenorepvec, Ad5/3-E2F-D24-hTNFα-IRES-hIL-2) is a chimeric oncolytic adenovirus suitable for intravenous (i.v.) delivery due to its capsid modification and dual selectivity devices. It is armed with tumor necrosis alpha and interleukin-2 for promoting T-cell activation and lymphocyte trafficking to tumors, thereby enhancing the antitumor immune response. Here, we present the findings after a single i.v. administration of TILT-123 in three phase I dose escalation clinical trials.

Methods: Patients with advanced solid tumors initially received a single i.v. dose of TILT-123 ranging from 3 × 109 to 4 × 1012 viral particles (VP). Blood was collected at baseline, 1, 16, and 192 h (7 days) post-treatment for bioavailability and serum analysis. Tumor biopsies were collected prior to treatment and 7 days post-treatment for analysis of viral presence and immunological effects. Patients did not receive any other cancer therapies during this period.

Results: Across all three trials (TUNIMO, TUNINTIL, and PROTA), 52 total patients were treated with i.v. TILT-123. Overall, TILT-123 was found to be well-tolerated, with no dose-limiting toxicities observed. Post-treatment tumor biopsies showed expression of viral genes, presence of TILT-123 adenovirus proteins or DNA, and changes in immune cell infiltration from baseline. Increased virus dose did not lead to increased virus detection in tumors. Median overall survival was longer in patients with confirmed presence of TILT-123 in post-treatment biopsies (280 versus 190 days, p = 0.0405).

Conclusion: TILT-123 demonstrated safety and significant intratumoral immunomodulation following a single i.v. administration, warranting further investigation.

Trial registrations: TUNIMO-NCT04695327. Registered 4 January 2021, https://clinicaltrials.gov/study/NCT04695327 . TUNINTIL-NCT04217473. Registered 19 December 2019, https://clinicaltrials.gov/study/NCT04217473 . PROTA-NCT05271318. Registered 4 February 2022, https://clinicaltrials.gov/study/NCT05271318 .

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来源期刊
CiteScore
18.20
自引率
1.80%
发文量
333
审稿时长
1 months
期刊介绍: The Journal of Experimental & Clinical Cancer Research is an esteemed peer-reviewed publication that focuses on cancer research, encompassing everything from fundamental discoveries to practical applications. We welcome submissions that showcase groundbreaking advancements in the field of cancer research, especially those that bridge the gap between laboratory findings and clinical implementation. Our goal is to foster a deeper understanding of cancer, improve prevention and detection strategies, facilitate accurate diagnosis, and enhance treatment options. We are particularly interested in manuscripts that shed light on the mechanisms behind the development and progression of cancer, including metastasis. Additionally, we encourage submissions that explore molecular alterations or biomarkers that can help predict the efficacy of different treatments or identify drug resistance. Translational research related to targeted therapies, personalized medicine, tumor immunotherapy, and innovative approaches applicable to clinical investigations are also of great interest to us. We provide a platform for the dissemination of large-scale molecular characterizations of human tumors and encourage researchers to share their insights, discoveries, and methodologies with the wider scientific community. By publishing high-quality research articles, reviews, and commentaries, the Journal of Experimental & Clinical Cancer Research strives to contribute to the continuous improvement of cancer care and make a meaningful impact on patients' lives.
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