用于检测淋病奈瑟菌的无标记液晶-液滴光学传感平台

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2024-11-03 DOI:10.1016/j.molliq.2024.126410

{"title":"用于检测淋病奈瑟菌的无标记液晶-液滴光学传感平台","authors":"","doi":"10.1016/j.molliq.2024.126410","DOIUrl":null,"url":null,"abstract":"<div><div>An oligonucleotide decorated liquid crystal (LC) droplet platform was studied for the detection of sexually transmitted disease (STD) caused by bacterium Neisseria gonorrhoeae. Homeotropically aligned nematic LC droplets were patterned on the silane modified glass plates and were utilized as sensing platform using an oligonucleotide probe (ssDNAprobe) sequence specific for N. gonorrhoeae. The developed LC-droplets platform could detect the synthetic complementary DNA (ssDNAtarget) and genomic DNA of N. gonorrhoeae with dark polarizing optical microscopy (POM) images of homeotropic LC alignment up to the lowest detectable limit of 10 pM and 50 pM, respectively. The DNA hybridization event was further monitored by zeta potential (ζ) measurement of LC droplets using dynamic light scattering technique to understand the sensing mechanism of ssDNAprobe hybridization with ssDNAtarget. The zeta potential response showed a linear relationship to ssDNAtarget over a concentration range of 10 pM to 100 nM and a limit of detection of 10 pM, which is nearly close to limit of detection (LOD) obtained with LC optical response measurement. These results indicated a similar kind of sensing mechanism with LC-droplets in both aqueous medium as well as on a glass substrate. The specificity of the LC droplet platform was investigated with various levels of mismatched synthetic DNA sequences and non-specific genomic DNA isolated from other bacteria. The homeotropic LC optical response with genomic DNA of N. gonorrhoeae in the presence of potential interferents showed that LC-droplet platform is highly selective for N. gonorrhoeae, suggesting a simple, rapid and reliable label-free detection platform.</div></div>","PeriodicalId":371,"journal":{"name":"Journal of Molecular Liquids","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A label-free liquid–crystal-droplet based optical sensing platform for the detection of Neisseria gonorrhoeae\",\"authors\":\"\",\"doi\":\"10.1016/j.molliq.2024.126410\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>An oligonucleotide decorated liquid crystal (LC) droplet platform was studied for the detection of sexually transmitted disease (STD) caused by bacterium Neisseria gonorrhoeae. Homeotropically aligned nematic LC droplets were patterned on the silane modified glass plates and were utilized as sensing platform using an oligonucleotide probe (ssDNAprobe) sequence specific for N. gonorrhoeae. The developed LC-droplets platform could detect the synthetic complementary DNA (ssDNAtarget) and genomic DNA of N. gonorrhoeae with dark polarizing optical microscopy (POM) images of homeotropic LC alignment up to the lowest detectable limit of 10 pM and 50 pM, respectively. The DNA hybridization event was further monitored by zeta potential (ζ) measurement of LC droplets using dynamic light scattering technique to understand the sensing mechanism of ssDNAprobe hybridization with ssDNAtarget. The zeta potential response showed a linear relationship to ssDNAtarget over a concentration range of 10 pM to 100 nM and a limit of detection of 10 pM, which is nearly close to limit of detection (LOD) obtained with LC optical response measurement. These results indicated a similar kind of sensing mechanism with LC-droplets in both aqueous medium as well as on a glass substrate. The specificity of the LC droplet platform was investigated with various levels of mismatched synthetic DNA sequences and non-specific genomic DNA isolated from other bacteria. The homeotropic LC optical response with genomic DNA of N. gonorrhoeae in the presence of potential interferents showed that LC-droplet platform is highly selective for N. gonorrhoeae, suggesting a simple, rapid and reliable label-free detection platform.</div></div>\",\"PeriodicalId\":371,\"journal\":{\"name\":\"Journal of Molecular Liquids\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-11-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Molecular Liquids\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167732224024693\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Liquids","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167732224024693","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

摘要

研究了一种寡核苷酸装饰液晶（LC）液滴平台，用于检测由淋病奈瑟氏菌引起的性传播疾病（STD）。在硅烷修饰的玻璃板上图案化了同向排列的向列液晶液滴，并使用针对淋病奈瑟菌的特异性寡核苷酸探针（ssDNAprobe）序列将其用作传感平台。所开发的液相色谱-液滴平台可以检测到合成互补 DNA（ssDNAtarget）和淋球菌基因组 DNA，其同源液相色谱排列的暗偏振光学显微镜（POM）图像的最低检测限分别为 10 pM 和 50 pM。利用动态光散射技术测量 LC 液滴的 zeta 电位 (ζ)，进一步监测 DNA 杂交事件，以了解 ssDNAprobe 与 ssDNAtarget 杂交的传感机制。在 10 pM 至 100 nM 的浓度范围内，zeta 电位响应与 ssDNAtarget 呈线性关系，检测限为 10 pM，几乎接近 LC 光学响应测量得到的检测限（LOD）。这些结果表明，液相色谱液滴在水介质和玻璃基底上都具有类似的传感机制。研究人员使用不同程度的不匹配合成 DNA 序列和从其他细菌中分离的非特异性基因组 DNA 对液相色谱液滴平台的特异性进行了研究。在存在潜在干扰物的情况下，淋球菌基因组 DNA 的同向液相色谱光学响应表明，液相色谱液滴平台对淋球菌具有高度选择性，是一种简单、快速、可靠的无标记检测平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A label-free liquid–crystal-droplet based optical sensing platform for the detection of Neisseria gonorrhoeae

查看原文本刊更多论文

A label-free liquid–crystal-droplet based optical sensing platform for the detection of Neisseria gonorrhoeae

An oligonucleotide decorated liquid crystal (LC) droplet platform was studied for the detection of sexually transmitted disease (STD) caused by bacterium Neisseria gonorrhoeae. Homeotropically aligned nematic LC droplets were patterned on the silane modified glass plates and were utilized as sensing platform using an oligonucleotide probe (ssDNA_probe) sequence specific for N. gonorrhoeae. The developed LC-droplets platform could detect the synthetic complementary DNA (ssDNA_target) and genomic DNA of N. gonorrhoeae with dark polarizing optical microscopy (POM) images of homeotropic LC alignment up to the lowest detectable limit of 10 pM and 50 pM, respectively. The DNA hybridization event was further monitored by zeta potential (ζ) measurement of LC droplets using dynamic light scattering technique to understand the sensing mechanism of ssDNA_probe hybridization with ssDNA_target. The zeta potential response showed a linear relationship to ssDNA_target over a concentration range of 10 pM to 100 nM and a limit of detection of 10 pM, which is nearly close to limit of detection (LOD) obtained with LC optical response measurement. These results indicated a similar kind of sensing mechanism with LC-droplets in both aqueous medium as well as on a glass substrate. The specificity of the LC droplet platform was investigated with various levels of mismatched synthetic DNA sequences and non-specific genomic DNA isolated from other bacteria. The homeotropic LC optical response with genomic DNA of N. gonorrhoeae in the presence of potential interferents showed that LC-droplet platform is highly selective for N. gonorrhoeae, suggesting a simple, rapid and reliable label-free detection platform.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.