Tomotsumi Fujisawa*, Nozomi Tanaka, Jun Tamogami and Masashi Unno,
{"title":"Natronomonas pharaonis 的感官罗多普勒蛋白 II O 中间体的视网膜色团构型","authors":"Tomotsumi Fujisawa*, Nozomi Tanaka, Jun Tamogami and Masashi Unno, ","doi":"10.1021/acs.biochem.4c0042010.1021/acs.biochem.4c00420","DOIUrl":null,"url":null,"abstract":"<p >Sensory rhodopsin II (SRII) is a prototype photosensor that binds the retinal Schiff base chromophore. Upon photoabsorption, SRII is transformed into the signaling state, where two long-lived photointermediates are known to contribute. One is the M intermediate containing the deprotonated 13-<i>cis</i> chromophore, and the other is the O intermediate that is believed to have the protonated all-<i>trans</i> chromophore. The chromophore in the O intermediate is also thought to have the atypical 15-<i>syn</i> (C═N <i>cis</i>) configuration about the Schiff base moiety. In this communication, we study SRII from <i>Natronomonas pharaonis</i> (<i>Np</i>SRII) using Raman spectroscopy and find that the retinal chromophore configuration in the O intermediate is the 13-<i>cis</i>, 15-<i>anti</i> (C═N <i>trans</i>), contrary to the conventional notion. This result points out the revision of the chromophore structural changes underlying the long-lived signaling state of SRII.</p>","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Retinal Chromophore Configuration in the O Intermediate of Sensory Rhodopsin II from Natronomonas pharaonis\",\"authors\":\"Tomotsumi Fujisawa*, Nozomi Tanaka, Jun Tamogami and Masashi Unno, \",\"doi\":\"10.1021/acs.biochem.4c0042010.1021/acs.biochem.4c00420\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Sensory rhodopsin II (SRII) is a prototype photosensor that binds the retinal Schiff base chromophore. Upon photoabsorption, SRII is transformed into the signaling state, where two long-lived photointermediates are known to contribute. One is the M intermediate containing the deprotonated 13-<i>cis</i> chromophore, and the other is the O intermediate that is believed to have the protonated all-<i>trans</i> chromophore. The chromophore in the O intermediate is also thought to have the atypical 15-<i>syn</i> (C═N <i>cis</i>) configuration about the Schiff base moiety. In this communication, we study SRII from <i>Natronomonas pharaonis</i> (<i>Np</i>SRII) using Raman spectroscopy and find that the retinal chromophore configuration in the O intermediate is the 13-<i>cis</i>, 15-<i>anti</i> (C═N <i>trans</i>), contrary to the conventional notion. This result points out the revision of the chromophore structural changes underlying the long-lived signaling state of SRII.</p>\",\"PeriodicalId\":28,\"journal\":{\"name\":\"Biochemistry Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry Biochemistry\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.biochem.4c00420\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry Biochemistry","FirstCategoryId":"1","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.biochem.4c00420","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
感光性视网膜红蛋白 II（SRII）是一种原型光传感器，能与视网膜希夫碱发色团结合。光吸收后，SRII 转变为信号状态，已知有两种长效光中间体在其中起作用。一种是含有去质子化 13 顺式发色团的 M 中间体，另一种是据认为含有质子化全反式发色团的 O 中间体。O 中间体中的发色团也被认为具有关于席夫碱分子的非典型 15-顺式（C═N 顺式）构型。在这篇通讯中，我们利用拉曼光谱研究了 Natronomonas pharaonis 的 SRII（NpSRII），发现 O 中间体中的视网膜发色团构型是 13-顺式、15-反式（C═N 反式），这与传统观念相反。这一结果指出了 SRII 长寿命信号状态背后的发色团结构变化的修正。
Retinal Chromophore Configuration in the O Intermediate of Sensory Rhodopsin II from Natronomonas pharaonis
Sensory rhodopsin II (SRII) is a prototype photosensor that binds the retinal Schiff base chromophore. Upon photoabsorption, SRII is transformed into the signaling state, where two long-lived photointermediates are known to contribute. One is the M intermediate containing the deprotonated 13-cis chromophore, and the other is the O intermediate that is believed to have the protonated all-trans chromophore. The chromophore in the O intermediate is also thought to have the atypical 15-syn (C═N cis) configuration about the Schiff base moiety. In this communication, we study SRII from Natronomonas pharaonis (NpSRII) using Raman spectroscopy and find that the retinal chromophore configuration in the O intermediate is the 13-cis, 15-anti (C═N trans), contrary to the conventional notion. This result points out the revision of the chromophore structural changes underlying the long-lived signaling state of SRII.
期刊介绍：
Biochemistry provides an international forum for publishing exceptional, rigorous, high-impact research across all of biological chemistry. This broad scope includes studies on the chemical, physical, mechanistic, and/or structural basis of biological or cell function, and encompasses the fields of chemical biology, synthetic biology, disease biology, cell biology, nucleic acid biology, neuroscience, structural biology, and biophysics. In addition to traditional Research Articles, Biochemistry also publishes Communications, Viewpoints, and Perspectives, as well as From the Bench articles that report new methods of particular interest to the biological chemistry community.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
