枸杞果胶多糖生物合成的酵素再现和基因组洞察。

Haiyan Yue, Yiheng Tang, Aixuan Li, Lili Zhang, Yiwei Niu, Yiming Zhang, Hao Wang, Jianjun Luo, Yi Zhao, Shunmin He, Chang Chen, Runsheng Chen
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引用次数: 0

摘要

枸杞是茄科植物,是重要的茄科植物,具有食药同源的特点。枸杞果胶多糖(LBPPs)是枸杞的主要生物活性成分,也是少数同时具有生物相容性和生物医学活性的多糖之一。以往的研究主要集中在枸杞多糖的功能特性上,但对其生物合成和通过关键酶运输的机制仍然知之甚少。在此,我们报告了枸杞 2.18 千兆位基准基因组的完成情况,首次重建了果胶多糖的整个生物合成和糖运输过程,并对负责果胶多糖骨架延伸、侧链合成和修饰的重要基因进行了表征。此外,我们还鉴定了与多糖代谢相关的长非编码 RNA(lncRNA),并发现了一种特异性鼠李糖半乳糖醛酸 I(RG-I)鼠李糖基转移酶 RRT3020,它能增强枸杞多糖中 RG-I 的生物合成。这些新发现的酶和关键基因赋予了 L. barbarum 特定的果胶生物合成能力,使其有别于其他茄科植物。我们的发现为进化研究和分子育种提供了基础,以提高枸杞的多样化应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enzymes Repertoires and Genomic Insights into Lycium Barbarum Pectin Polysaccharides Biosynthesis.

Lycium barbarum, a member of the Solanaceae family, represents an important eudicot lineage with homology of food and medicine. Lycium barbarum pectin polysaccharides (LBPPs) are key bioactive ingredients of Lycium barbarum, and are among the few polysaccharides with both biocompatibility and biomedical activity. While previous studies have primarily focused on the functional properties of LBPPs, the mechanisms of biosynthesis and transport by key enzymes remain poorly understood. Here, we reported the completion of a 2.18-gigabase reference genome of Lycium barbarum, reconstructed the first entire biosynthesis of pectin polysaccharides and sugar transport, and characterized the important genes responsible for backbone extending, sidechain synthesis, and modification of pectin polysaccharides. Additionally, we characterized long non-coding RNAs (lncRNAs) associated with polysaccharide metabolism and identified a specific rhamnogalacturonan I (RG-I) rhamnosyltransferase, RRT3020, which enhances RG-I biosynthesis in LBPPs. These newly identified enzymes and pivotal genes endow L. barbarum with specific pectin biosynthesis capabilities, distinguishing it from other Solanaceae species. Our findings provide a foundation for evolutionary studies and molecular breeding to enhance the diverse applications of L. barbarum.

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