{"title":"利用实用的荧光测定法解读锌在炎症中对抗氧化应激的潜在作用。","authors":"Zengyan Lin, Lanlan Zhang, Daliang Li","doi":"10.1016/j.bioorg.2024.107886","DOIUrl":null,"url":null,"abstract":"<p><p>Zinc plays a critical role in inflammation and apoptosis, potentially offering new insights into health and disease beyond its established involvement in various biological processes. A fluorescent probe, SPI, has been designed and synthesized for the real-time detection of dynamic changes of zinc ions (Zn<sup>2+</sup>) in the potential resistance to oxidative stress, showing fluorescence enhancement at approximately 639 nm with a limit of detection of around 65 pM, which allowed it to identify even low concentrations of Zn<sup>2+</sup> with intrinsic excellent biocompatibility. By establishing a cellular inflammation and apoptosis model using HT-DNA, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and dexamethasone (DXMS), the study effectively simulates conditions that can alter Zn<sup>2+</sup> dynamics. Monitoring the fluorescence changes of SPI in response to these conditions allows researchers to observe how Zn<sup>2+</sup> levels fluctuate in real-time, providing a clearer picture of its role in maintaining intracellular redox homeostasis. The findings indicate that SPI can be instrumental in elucidating the detailed molecular mechanisms through which Zn<sup>2+</sup> influences immune responses and associates with cellular stress pathways. Overall, the development of SPI not only replenishes a potential assay into the toolbox to study Zn<sup>2+</sup> in living cells but also opens new avenues for the further investigations into the therapeutic potential of modulating zinc levels in various pathological conditions.</p>","PeriodicalId":257,"journal":{"name":"Bioorganic Chemistry","volume":"153 ","pages":"107886"},"PeriodicalIF":4.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Interpret the potential role of zinc against oxidative stress in inflammation with a practical fluorescent assay.\",\"authors\":\"Zengyan Lin, Lanlan Zhang, Daliang Li\",\"doi\":\"10.1016/j.bioorg.2024.107886\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Zinc plays a critical role in inflammation and apoptosis, potentially offering new insights into health and disease beyond its established involvement in various biological processes. A fluorescent probe, SPI, has been designed and synthesized for the real-time detection of dynamic changes of zinc ions (Zn<sup>2+</sup>) in the potential resistance to oxidative stress, showing fluorescence enhancement at approximately 639 nm with a limit of detection of around 65 pM, which allowed it to identify even low concentrations of Zn<sup>2+</sup> with intrinsic excellent biocompatibility. By establishing a cellular inflammation and apoptosis model using HT-DNA, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and dexamethasone (DXMS), the study effectively simulates conditions that can alter Zn<sup>2+</sup> dynamics. Monitoring the fluorescence changes of SPI in response to these conditions allows researchers to observe how Zn<sup>2+</sup> levels fluctuate in real-time, providing a clearer picture of its role in maintaining intracellular redox homeostasis. The findings indicate that SPI can be instrumental in elucidating the detailed molecular mechanisms through which Zn<sup>2+</sup> influences immune responses and associates with cellular stress pathways. Overall, the development of SPI not only replenishes a potential assay into the toolbox to study Zn<sup>2+</sup> in living cells but also opens new avenues for the further investigations into the therapeutic potential of modulating zinc levels in various pathological conditions.</p>\",\"PeriodicalId\":257,\"journal\":{\"name\":\"Bioorganic Chemistry\",\"volume\":\"153 \",\"pages\":\"107886\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioorganic Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.bioorg.2024.107886\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/23 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioorganic Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.bioorg.2024.107886","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/23 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Interpret the potential role of zinc against oxidative stress in inflammation with a practical fluorescent assay.
Zinc plays a critical role in inflammation and apoptosis, potentially offering new insights into health and disease beyond its established involvement in various biological processes. A fluorescent probe, SPI, has been designed and synthesized for the real-time detection of dynamic changes of zinc ions (Zn2+) in the potential resistance to oxidative stress, showing fluorescence enhancement at approximately 639 nm with a limit of detection of around 65 pM, which allowed it to identify even low concentrations of Zn2+ with intrinsic excellent biocompatibility. By establishing a cellular inflammation and apoptosis model using HT-DNA, hydrogen peroxide (H2O2), and dexamethasone (DXMS), the study effectively simulates conditions that can alter Zn2+ dynamics. Monitoring the fluorescence changes of SPI in response to these conditions allows researchers to observe how Zn2+ levels fluctuate in real-time, providing a clearer picture of its role in maintaining intracellular redox homeostasis. The findings indicate that SPI can be instrumental in elucidating the detailed molecular mechanisms through which Zn2+ influences immune responses and associates with cellular stress pathways. Overall, the development of SPI not only replenishes a potential assay into the toolbox to study Zn2+ in living cells but also opens new avenues for the further investigations into the therapeutic potential of modulating zinc levels in various pathological conditions.
期刊介绍:
Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry.
For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature.
The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.