布氏锥虫应激颗粒的动态组成

IF 5.5 1区 医学 Q1 MICROBIOLOGY
PLoS Pathogens Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.1371/journal.ppat.1012666
Htay Mon Aye, Feng-Jun Li, Cynthia Y He
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引用次数: 0

摘要

应激颗粒(SGs)是应激诱导的 RNA 凝聚物,由因翻译抑制而停滞的起始复合物组成。应激颗粒的生化组成和功能多种多样,这种多样性可归因于不同的应激条件、所涉及的信号通路和特定的细胞类型。有趣的是,在被称为加工体(PB)的无处不在的细胞质病灶中发现的 mRNA 衰减成分也在 SG 蛋白组中被发现。目前 SG 研究的一大挑战是了解 SG 多样性的原因以及 SG 在不同应激条件下的功能。布氏锥虫(Trypanosoma brucei)是一种单细胞寄生虫,可导致人类非洲锥虫病(sleping sickness)。在这项研究中,我们发现在饥饿状态下,通过改变细胞外碳源的供应,细胞内的 ATP 水平会迅速发生变化,从而产生不同组成和动态的 SG。我们发现了一部分 SG 成分,它们在细胞 ATP 枯竭时从 SG 中分离出来。利用膨胀显微镜,我们观察到了应力颗粒内 PB 和 SG 成分的亚颗粒区隔。我们的研究结果突显了细胞 ATP 在 SG 组成和动态中的重要性,为在不同应激条件下形成的 SG 提供了功能性见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic composition of stress granules in Trypanosoma brucei.

Stress granules (SGs) are stress-induced RNA condensates consisting of stalled initiation complexes resulting from translational inhibition. The biochemical composition and function of SGs are highly diverse, and this diversity has been attributed to different stress conditions, signalling pathways involved and specific cell types. Interestingly, mRNA decay components, which are found in ubiquitous cytoplasmic foci known as processing bodies (PB), have also been identified in SG proteomes. A major challenge in current SG studies is to understand the cause of SG diversity, as well as the function of SG under different stress conditions. Trypanosoma brucei is a single-cellular parasite that causes Human African Trypanosomiasis (sleeping sickness). In this study, we showed that by varying the supply of extracellular carbon sources during starvation, cellular ATP levels changed rapidly, resulting in SGs of different compositions and dynamics. We identified a subset of SG components, which dissociated from the SGs in response to cellular ATP depletion. Using expansion microscopy, we observed sub-granular compartmentalization of PB- and SG-components within the stress granules. Our results highlight the importance of cellular ATP in SG composition and dynamics, providing functional insight to SGs formed under different stress conditions.

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来源期刊
PLoS Pathogens
PLoS Pathogens MICROBIOLOGY-PARASITOLOGY
自引率
3.00%
发文量
598
期刊介绍: Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.
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