Wei Xiao, Qing Zhang, Dong Hui You, Nian Bing Li, Guang Ming Zhou, Hong Qun Luo
{"title":"基于黄酮醇-苯并噻唑的 FRET 探针,用于检测粘度和二氧化硫衍生物。","authors":"Wei Xiao, Qing Zhang, Dong Hui You, Nian Bing Li, Guang Ming Zhou, Hong Qun Luo","doi":"10.1016/j.bioorg.2024.107913","DOIUrl":null,"url":null,"abstract":"<p><p>Sulfur dioxide (SO<sub>2</sub>) and viscosity play important roles in living organisms, and abnormal levels of them are associated with many diseases. Hence, a bifunctional fluorescence probe (E)-3-(2-(4-(4-(4-(6-fluoro-3-hydroxy-4-oxo-4H-chromen-2-yl)benzoyl)piperazin-1-yl)styryl)benzo-[d]thiazol-3-ium-3-yl)propane-1-sulfonate (HFBT) with fluorescence resonance energy transfer (FRET) properties was successfully constructed by using 3-hydroxyflavonol as the energy donor and benzothiazole sulphonate derivatives as the energy acceptor, and it can be used for the detection of SO<sub>2</sub> derivatives (HSO<sub>3</sub><sup>-</sup>/HSO<sub>3</sub><sup>2-</sup>) and viscosity. HFBT exhibits a large Stokes shift (245 nm), high resonance energy transfer efficiency (95.56 %), and excellent selectivity, anti-interference and low limit of detection (LOD = 0.057 μM) for HSO<sub>3</sub><sup>-</sup>. The fluorescence intensity of HFBT at 596 nm gradually increases with the increase of viscosity. Interestingly, a visual HSO<sub>3</sub><sup>-</sup> detection platform was successfully constructed and applied to the quantitative detection of HSO<sub>3</sub><sup>-</sup> in food. Additionally, HFBT was successfully applied to imaging endogenous and exogenous HSO<sub>3</sub><sup>-</sup> in cells. The successful development of HFBT provides an effective tool for the detection and imaging of HSO<sub>3</sub><sup>-</sup> in food and cells.</p>","PeriodicalId":257,"journal":{"name":"Bioorganic Chemistry","volume":"153 ","pages":"107913"},"PeriodicalIF":4.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A FRET probe based on flavonol-benzothiazole for the detection of viscosity and SO<sub>2</sub> derivatives.\",\"authors\":\"Wei Xiao, Qing Zhang, Dong Hui You, Nian Bing Li, Guang Ming Zhou, Hong Qun Luo\",\"doi\":\"10.1016/j.bioorg.2024.107913\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sulfur dioxide (SO<sub>2</sub>) and viscosity play important roles in living organisms, and abnormal levels of them are associated with many diseases. Hence, a bifunctional fluorescence probe (E)-3-(2-(4-(4-(4-(6-fluoro-3-hydroxy-4-oxo-4H-chromen-2-yl)benzoyl)piperazin-1-yl)styryl)benzo-[d]thiazol-3-ium-3-yl)propane-1-sulfonate (HFBT) with fluorescence resonance energy transfer (FRET) properties was successfully constructed by using 3-hydroxyflavonol as the energy donor and benzothiazole sulphonate derivatives as the energy acceptor, and it can be used for the detection of SO<sub>2</sub> derivatives (HSO<sub>3</sub><sup>-</sup>/HSO<sub>3</sub><sup>2-</sup>) and viscosity. HFBT exhibits a large Stokes shift (245 nm), high resonance energy transfer efficiency (95.56 %), and excellent selectivity, anti-interference and low limit of detection (LOD = 0.057 μM) for HSO<sub>3</sub><sup>-</sup>. The fluorescence intensity of HFBT at 596 nm gradually increases with the increase of viscosity. Interestingly, a visual HSO<sub>3</sub><sup>-</sup> detection platform was successfully constructed and applied to the quantitative detection of HSO<sub>3</sub><sup>-</sup> in food. Additionally, HFBT was successfully applied to imaging endogenous and exogenous HSO<sub>3</sub><sup>-</sup> in cells. The successful development of HFBT provides an effective tool for the detection and imaging of HSO<sub>3</sub><sup>-</sup> in food and cells.</p>\",\"PeriodicalId\":257,\"journal\":{\"name\":\"Bioorganic Chemistry\",\"volume\":\"153 \",\"pages\":\"107913\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioorganic Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.bioorg.2024.107913\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioorganic Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.bioorg.2024.107913","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/24 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A FRET probe based on flavonol-benzothiazole for the detection of viscosity and SO2 derivatives.
Sulfur dioxide (SO2) and viscosity play important roles in living organisms, and abnormal levels of them are associated with many diseases. Hence, a bifunctional fluorescence probe (E)-3-(2-(4-(4-(4-(6-fluoro-3-hydroxy-4-oxo-4H-chromen-2-yl)benzoyl)piperazin-1-yl)styryl)benzo-[d]thiazol-3-ium-3-yl)propane-1-sulfonate (HFBT) with fluorescence resonance energy transfer (FRET) properties was successfully constructed by using 3-hydroxyflavonol as the energy donor and benzothiazole sulphonate derivatives as the energy acceptor, and it can be used for the detection of SO2 derivatives (HSO3-/HSO32-) and viscosity. HFBT exhibits a large Stokes shift (245 nm), high resonance energy transfer efficiency (95.56 %), and excellent selectivity, anti-interference and low limit of detection (LOD = 0.057 μM) for HSO3-. The fluorescence intensity of HFBT at 596 nm gradually increases with the increase of viscosity. Interestingly, a visual HSO3- detection platform was successfully constructed and applied to the quantitative detection of HSO3- in food. Additionally, HFBT was successfully applied to imaging endogenous and exogenous HSO3- in cells. The successful development of HFBT provides an effective tool for the detection and imaging of HSO3- in food and cells.
期刊介绍:
Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry.
For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature.
The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.