Prostanoid synthesis in isolated parenchymal and nonparenchymal mouse liver cells in the presence of arachioonic acid

Prostanoid synthesis was investigated in suspensions of isolated mouse hepatocytes and nonparenchymal liver cells. A stable metabolite of thromboxane A₂ (TXB₂) of prostacyclin (6-keto PGF_1∝) and one of the prostaglandins (PGF_2∝) was detected by radio-immuno-assay (RIA). Hepatocytes synthesized mainly TXB₂, while smaller amounts of 6-keto PGF_1∝ and PGF_2∝ were detected during 60 min incubation. Homogenization of hepatocytes caused a slight increase in TXB₂ production and provoked the synthesis of PGF_2∝ and 6-keto PGF_1∝. Theaddition ofarachidonate to hepatocytes did not influence prostanoid production at concentrations below 10⁻⁵ M. Higher concentrations further increased TXB₂ production and also increased the synthesis of 6-keto PGF_1∝ and PGF_2∝. Nonparenchymal cells synthesized all the three types of prostanoids and homogenization of these cells did not result in a marked change. The addition of 10⁻⁷−10⁻⁵M arachidonate increased the TXB₂, 6-keto PGF_1∝ and PGF_2∝ synthesis in nonparenchymal cells. No further increase was found at higher concentrations.