玉米激素反应测定的卷纸法

Cold Spring Harbor protocols Pub Date : 2024-10-30 DOI:10.1101/pdb.prot108623

Clarice F Gonzales, Craig L Cowling, Dior R Kelley

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引用次数: 0

摘要

卷巾试验（RTA）是一种评估玉米和大豆等作物幼苗表型的无土方法。在此，我们提供了一种基于 RTA 的最新方案，用于对玉米幼苗对相关化学品的反应进行表型分析。我们以两种合成的辅助素除草剂（2,4-二氯苯氧乙酸和毒莠定）、一种辅助素前体（吲哚-3-丁酸）和一种辅助素抑制剂（N-1-萘酞胺酸）为示例，但该方法也可用于其他可溶于生长介质的激素或植物生长调节剂。我们还提供了如何注释根系性状和分析主根长度性状数据的说明。该方案可扩展用于基因筛选、为基因表达分析准备组织、开展全基因组关联研究（GWAS）以及数量性状基因座（QTL）鉴定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Rolled Towel Method for Hormone Response Assays in Maize.

The rolled towel assay (RTA) is a soil-free method to evaluate juvenile phenotypes in crops such as maize and soybean. Here, we provide an updated RTA-based protocol to phenotype maize seedling responses to chemicals of interest. We exemplify the protocol with two synthetic auxin herbicides (2,4-dichlorophenoxyacetic acid and picloram), an auxin precursor (indole-3-butyric acid), and an auxin inhibitor (N-1-naphthylphthalamic acid), but the method can be used with other hormones or plant growth regulators that are soluble in growth media. We also include instructions on how to annotate root traits and analyze primary root length trait data. The protocol can be scaled up for use in genetic screens, preparing tissue for gene expression analyses, carrying out genome-wide association studies (GWASs), and quantitative trait locus (QTL) identification.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.