球孢子菌膜囊泡破坏了致龋生物界间的组合。

IF 3.9 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Applied and Environmental Microbiology Pub Date : 2024-11-20 Epub Date: 2024-10-31 DOI:10.1128/aem.00885-24
Puthayalai Treerat, Tanner Rozendal, Camilla de Mattos, Anli Davis, Emily Helliwell, Justin Merritt, Jens Kreth
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引用次数: 0

摘要

牙周病和龋齿等多微生物疾病对抗生素治疗等常见方法具有抗药性,给治疗带来了巨大挑战。由于微生物之间的相互作用也会破坏宿主的免疫反应,这些感染表现出更强的恢复力。目前的研究重点是毒力和促进疾病的相互作用,但对可抑制或预防疾病发展的相互作用却知之甚少。正常情况下,与人类相关的微生物群会保持平衡,防止病原菌占据主导地位。在慢性播散性念珠菌病或严重儿童早期龋齿(s-ECC)等疾病中,白色念珠菌等微生物的过度生长会破坏这种平衡。通常情况下,白色念珠菌在微生物群落中良性共存,但也可能成为致病菌,形成生物膜并与其他微生物(如致龋的变异链球菌)相互作用。这种相互作用对 s-ECC 尤为重要,它会加剧疾病的发展和严重程度。在这里,我们发现棒状杆菌本身以及通过其细胞外膜囊泡破坏了白喉杆菌和变异链球菌之间的相互作用。从机理上讲,这种相互作用的干扰发生在基因水平上,HWP1 的表达下调,这种表面蛋白在突变酵母菌存在时会被特异性诱导,从而促进它们之间的相互作用。此外,我们还发现,杜兰球菌能阻碍白僵菌在麦角菌感染模型中的系统毒性。这表明口腔棒状杆菌可能是一种有益的共生物种,在多微生物群落中发挥抗真菌作用,并为管理多微生物疾病开辟了新途径。要预防这种疾病,就必须在疾病症状出现之前深入了解生态过程,这可能是一种潜在的策略。在这种情况下,我们研究了与口腔健康相关的相对丰富的口腔生物膜科里奈杆菌如何干扰变异链球菌和白色念珠菌的跨王国伙伴关系。这种合作关系是 s-ECC 龋齿的重要驱动因素,因为它们的协同活动会增加致龋性。我们的研究发现,口腔球杆菌通过产生胞外膜囊泡,可以抑制真菌菌丝的形成,从而破坏变异球菌和白念珠菌的合作关系。此外,这些囊泡中的脂肪酸货物还具有抗真菌特性,这表明棒状杆菌在塑造口腔生物膜内的微生物动态方面发挥了作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Corynebacterial membrane vesicles disrupt cariogenic interkingdom assemblages.

Polymicrobial diseases such as periodontal disease and caries pose significant treatment challenges due to their resistance to common approaches like antibiotic therapy. These infections exhibit increased resilience, due to microbial interactions that also disrupt host immune responses. Current research focuses on virulence and disease-promoting interactions, but less is known about interactions that could inhibit or prevent disease development. Normally human-associated microbiomes maintain homeostasis, preventing pathobionts from becoming dominant. In conditions like chronic disseminated candidiasis or severe early childhood caries (s-ECC), an overgrowth of microbes such as Candida albicans disrupts this balance. Typically, C. albicans coexists benignly within the microbial community but can become pathogenic, forming biofilms and interacting with other microbes such as cariogenic Streptococcus mutans. This interaction is particularly significant in s-ECC, where it exacerbates the disease's progression and severity. Here, we present that Corynebacterium durum, itself and through its extracellular membrane vesicles disrupts interkingdom assemblages between C. albicans and S. mutans. Mechanistically the interaction interference occurs at the genetic level with downregulated HWP1 expression, a surface protein specifically induced in the presence of S. mutans promoting the interkingdom interaction. Additionally, we show that C. durum can impede C. albicans systemic virulence in the Galleria mellonella infection model. This suggests that oral corynebacteria may act as a beneficial commensal species, exerting antifungal effects within polymicrobial communities and opening new avenues for managing polymicrobial diseases.IMPORTANCEPolymicrobial diseases such as severe early childhood caries (s-ECC) lack effective treatment options. Prevention, requiring a deeper understanding of ecological processes before the onset of disease symptoms, could be a potential strategy. In this context, we investigated how relatively abundant oral biofilm Corynebacterium species, which are associated with oral health, can interfere with the interkingdom partnership of Streptococcus mutans and Candida albicans. This partnership is a significant driver of tooth decay in s-ECC due to synergistic activities that increase cariogenicity. Our study reveals that oral corynebacteria, through the production of extracellular membrane vesicles, can disrupt the S. mutans and C. albicans partnership by inhibiting fungal hyphae formation. Additionally, the fatty acid cargo within these vesicles exhibits antifungal properties, suggesting that corynebacteria play a role in shaping microbial dynamics within the oral biofilm.

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来源期刊
Applied and Environmental Microbiology
Applied and Environmental Microbiology 生物-生物工程与应用微生物
CiteScore
7.70
自引率
2.30%
发文量
730
审稿时长
1.9 months
期刊介绍: Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.
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