荧光光谱在解读核酸动力学方面的生物物理意义：从基础到最新进展

IF 3.3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biophysical chemistry Pub Date : 2024-10-22 DOI:10.1016/j.bpc.2024.107345

Vivek Pandey , Tejasvi Pandey

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引用次数: 0

摘要

荧光光谱学彻底改变了核酸研究，为了解基因表达、复制和修复的动态过程提供了宝贵的见解。本综述探讨了荧光探针在监测 DNA 和 RNA 的构象变化、相互作用和调控机制方面的应用。我们讨论了插层和非插层荧光探针在实时跟踪核酸动态中的应用，强调了它们在阐明 DNA 复制、转录调控和 DNA 修复的分子机制中的作用。通过对最新进展的详细分析，这篇综述强调了基于荧光的技术在加深我们对核酸行为及其对基因组稳定性和治疗干预的影响的理解方面所具有的重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Biophysical significance of fluorescence spectroscopy in deciphering nucleic acid dynamics: From fundamental to recent advancements

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Biophysical significance of fluorescence spectroscopy in deciphering nucleic acid dynamics: From fundamental to recent advancements

Fluorescence spectroscopy has revolutionized the study of nucleic acids, providing invaluable insights into the dynamic processes that underpin gene expression, replication, and repair. This review explores the application of fluorescence probes in monitoring the conformational changes, interactions, and regulatory mechanisms of DNA and RNA. We discuss the utility of intercalating and non-intercalating fluorescent probes in real-time tracking of nucleic acid dynamics, highlighting their role in elucidating the molecular mechanisms of DNA replication, transcriptional regulation, and DNA repair. By offering a detailed analysis of recent advancements, this review underscores the significance of fluorescence-based techniques in enhancing our understanding of nucleic acid behavior and their implications for genomic stability and therapeutic interventions.

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来源期刊

Biophysical chemistry 生物-生化与分子生物学

CiteScore

6.10

自引率

10.50%

发文量

121

审稿时长

20 days

期刊介绍： Biophysical Chemistry publishes original work and reviews in the areas of chemistry and physics directly impacting biological phenomena. Quantitative analysis of the properties of biological macromolecules, biologically active molecules, macromolecular assemblies and cell components in terms of kinetics, thermodynamics, spatio-temporal organization, NMR and X-ray structural biology, as well as single-molecule detection represent a major focus of the journal. Theoretical and computational treatments of biomacromolecular systems, macromolecular interactions, regulatory control and systems biology are also of interest to the journal.