基于结构转换触发模拟酶活性的比率荧光传感器，用于灵敏检测恩诺沙星

IF 2.8 3区化学 Q3 CHEMISTRY, PHYSICAL

Chemical Physics Letters Pub Date : 2024-10-17 DOI:10.1016/j.cplett.2024.141684

Menglong Liu, Yiyao Liu, Jingyi Xiao, Yi Ren, Xue Gao

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引用次数: 0

摘要

基于碳点（CD）构建了一种结构转换触发的适配传感器，用于检测恩诺沙星（ENR）。由 CDs 功能化的适配体和两条由 hemin 单体修饰的互补链（cDNA1 和 cDNA2）自组装成传感器。hemin单体的过氧化物酶模拟活性可催化邻苯二胺氧化，生成具有黄色荧光的2,3-二氨基酚嗪（oxOPD）。同时，在发生荧光共振能量转移的过程中，CD 的荧光被淬灭。ENR 的检测线性范围为 0.1-12 ng mL-1，检测限为 40.7pg mL-1。此外，该灵敏传感器在检测实际水产品样品时表现出较高的准确性和重复性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Ratiometric fluorescence aptasensor based on structure-switching-triggered mimetic enzyme activity for sensitive detection of enrofloxacin

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Ratiometric fluorescence aptasensor based on structure-switching-triggered mimetic enzyme activity for sensitive detection of enrofloxacin

A structure-switching-triggered aptasensor was constructed based on carbon dots (CDs) to detect enrofloxacin (ENR). CDs-functionalized aptamer and two complementary chains (cDNA1 and cDNA2) modified by hemin monomer were self-assembled as sensor. The peroxidase-mimicking activity of hemin monomer can catalyze the oxidation of o-phenylenediamine to form 2,3-diaminophenazine (oxOPD) with yellow fluorescence. Simultaneously, during to the occurrence of fluorescence resonance energy transfer, the fluorescence of CDs was quenched. The detection linear range of ENR is 0.1–12 ng mL⁻¹, and the detection limit is 40.7pg mL⁻¹. Furthermore, the aptasensor demonstrated high accuracy and repeatability for the detection of actual aquatic product samples.

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来源期刊

Chemical Physics Letters 化学-物理：原子、分子和化学物理

CiteScore

5.70

自引率

3.60%

发文量

798

审稿时长

33 days

期刊介绍： Chemical Physics Letters has an open access mirror journal, Chemical Physics Letters: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Chemical Physics Letters publishes brief reports on molecules, interfaces, condensed phases, nanomaterials and nanostructures, polymers, biomolecular systems, and energy conversion and storage. Criteria for publication are quality, urgency and impact. Further, experimental results reported in the journal have direct relevance for theory, and theoretical developments or non-routine computations relate directly to experiment. Manuscripts must satisfy these criteria and should not be minor extensions of previous work.