利用 Sand-PDMS 薄膜装置控制冰核形成可提高小鼠前卵巢卵泡的冷冻保存效果。

IF 0.8 4区 医学 Q4 ENGINEERING, BIOMEDICAL
Samantha Stewart, Alisa White, Wenquan Ou, Wei Liu, Jennifer Nagashima, Nucharin Songsasen, Xiaoming He
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引用次数: 0

摘要

卵泡冷冻保存是一种很有前景的生育力保存策略;然而,冷冻保存方案仍有改进的余地,以最大限度地提高卵泡解冻后的存活率和质量。目前的缓慢冷冻方案要么使用人工植冰,要么使用昂贵的可编程速率冷冻机或其他临床上不兼容的冰启动器来控制细胞外溶液中的植冰温度,而这是影响冷冻保存后细胞/组织质量的关键参数。以前的研究表明,沙子是一种生物相容性极佳的冰引发剂,将其用于人类诱导多能干细胞的冷冻保存可使细胞在冷冻保存后具有较高的存活率和质量。本研究在小鼠模型中采用简单的缓慢冷冻方案,将沙子作为冰引发剂冷冻保存多细胞微组织--前卵巢卵泡。使用部分包埋在聚二甲基硅氧烷(PDMS)薄膜中的沙子在细胞外溶液中播撒冰种子进行冷冻保存的卵泡形态健康、存活率高,并能在解冻后与新鲜卵泡在培养液中相似地生长。这种基于沙子的冷冻保存策略可以方便地使用简单的设备进行卵泡冷冻保存,这项研究进一步证明了这种策略不仅适用于单细胞,也适用于多细胞组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Controlled Ice Nucleation With a Sand-PDMS Film Device Enhances Cryopreservation of Mouse Preantral Ovarian Follicles.

Ovarian follicle cryopreservation is a promising strategy for fertility preservation; however, cryopreservation protocols have room for improvement to maximize post-thaw follicle viability and quality. Current slow-freezing protocols use either manual ice-seeding in combination with expensive programmable-rate freezers or other clinically incompatible ice initiators to control the ice-seeding temperature in the extracellular solution, a critical parameter that impacts post-cryopreservation cell/tissue quality. Previously, sand has been shown to be an excellent, biocompatible ice initiator, and its use in cryopreservation of human induced pluripotent stem cells enables high cell viability and quality after cryopreservation. This study applies sand as an ice initiator to cryopreserve multicellular microtissue, preantral ovarian follicles, using a simple slow-freezing protocol in the mouse model. Ovarian follicles cryopreserved using the sand partially embedded in polydimethylsiloxane (PDMS) film to seed ice in the extracellular solution exhibit healthy morphology, high viability, and the ability to grow similarly to fresh follicles in culture post-thaw. This sand-based cryopreservation strategy can facilitate convenient ovarian follicle cryopreservation using simple equipment, and this study further demonstrates the translatability of this strategy to not only single cells but also multicellular tissues.

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来源期刊
CiteScore
1.80
自引率
11.10%
发文量
56
审稿时长
6-12 weeks
期刊介绍: The Journal of Medical Devices presents papers on medical devices that improve diagnostic, interventional and therapeutic treatments focusing on applied research and the development of new medical devices or instrumentation. It provides special coverage of novel devices that allow new surgical strategies, new methods of drug delivery, or possible reductions in the complexity, cost, or adverse results of health care. The Design Innovation category features papers focusing on novel devices, including papers with limited clinical or engineering results. The Medical Device News section provides coverage of advances, trends, and events.
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