与巴西圣弗朗西斯科盆地 H2 排放区相关的微生物组。

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY
Otávio Henrique Bezerra Pinto, Rafael da Silva Oliveira, Brendo Ramos Ferreira, Julianna Peixoto, Maria Regina Silveira Sartori, Betania Ferraz Quirino, Fabrice Brunet, Ricardo Henrique Kruger
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引用次数: 0

摘要

背景:二氢(H₂)天然气是一种清洁的可再生能源,在向可持续能源过渡的过程中备受关注。与传统的石油燃料不同,二氢天然气在燃烧时只释放水蒸气,这使其成为未来减少碳足迹的一种有前途的替代能源。然而,微生物对 H₂排放区内 H₂ 动力的影响以及 H2 的来源--是在更深处还是在浅层土壤中产生--仍不清楚。在圣弗朗西斯科盆地,在贫瘠的地面凹陷处发现土壤氢浓度约为 200 ppm。在这项研究中,我们使用 16S rRNA 基因测序法调查了与该地区相关的微生物群,重点研究了与氢₂消耗和产生相关的代谢过程。我们从两个监测点收集了土壤样本(结果见表 2):我们的研究结果表明,H2 排放区极大地影响了微生物群的组成和功能,芽孢杆菌是主要的微生物属。与典型的塞拉多(Cerrado)土壤相比,我们观察到放线菌群(∼ 40%)和固着菌群(∼ 20%)的流行率较高。此外,我们还发现了大量的孢子菌和以前被描述为 H2- 氧化菌的分类群:结论:圣弗朗西斯科盆地的 H2- 辐射区为我们提供了一个独特的机会,可借以加深了解 H₂ 对微生物群落的影响。这项研究首次采用独立于培养的方法描述了塞拉多土壤中与 H2- 相关的天然细菌群落的特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiome associated to an H2-emitting zone in the São Francisco basin Brazil.

Background: Dihydrogen (H₂) natural gas is a clean and renewable energy source of significant interest in the transition to sustainable energy. Unlike conventional petroleum-based fuels, H₂ releases only water vapor upon combustion, making it a promising alternative for reducing carbon footprints in the future. However, the microbial impact on H₂ dynamics in H2-emitting zones remains unclear, as does the origin of H2 - whether it is produced at greater depths or within shallow soil layers. In the São Francisco Basin, soil hydrogen concentrations of approximately 200 ppm were identified in barren ground depressions. In this study, we investigated the microbiome associated with this area using the 16S rRNA gene sequencing, with a focus on metabolic processes related to H₂ consumption and production. Soil samples were collected from two monitored (< 1 m) depths - 10 cm and 1 m - in the emission zone, which is predominantly covered with pasture vegetation, and from an adjacent area with medium and small trees.

Results: Our findings suggest that the H2-emitting zone significantly influences the composition and function of the microbiome, with Bacillus emerging as the dominant genus. In contrast to typical Cerrado soil, we observed a higher prevalence of Actinobacteriota (∼ 40%) and Firmicutes (∼ 20%). Additionally, we identified an abundance of sporulating bacteria and taxonomic groups previously described as H2-oxidizing bacteria.

Conclusions: The H2-emitting zone in the São Francisco Basin presents a unique opportunity to deepen our understanding of the impact of H₂ on microbial communities. This study is the first to characterize a natural H2-associated bacterial community in Cerrado soil using a culture-independent approach.

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来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
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